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Rabbit Recombinant Monoclonal IKB epsilon antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-IKB epsilon antibody [EPR901(2)] (AB134143), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB epsilon antibody [EPR901(2)] (AB134143), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Alternative names

IKBE, NFKBIE, NF-kappa-B inhibitor epsilon, NF-kappa-BIE, I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon

Recommended products

Rabbit Recombinant Monoclonal IKB epsilon antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR901(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-IKB epsilon antibody [EPR901(2)] (ab134143), expandable thumbnail

    Western blot - Anti-IKB epsilon antibody [EPR901(2)] (ab134143)

    All lanes: Western blot - Anti-IKB epsilon antibody [EPR901(2)] (ab134143) at 1/1000 dilution

    Lane 1: THP-1 cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: A375 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa

    Observed band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB epsilon antibody [EPR901(2)] (ab134143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB epsilon antibody [EPR901(2)] (ab134143)

    Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labelling IKB epsilon with ab134143 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com