Anti- IKK beta antibody [EPR16628]
- RabMAb
- Recombinant
- KO Validated
- 20ul selling size
- What is this?
4
(5 Reviews)
|
(70 Publications)
Rabbit Recombinant Monoclonal IKK beta antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 70 publications.
View Alternative Names
IKKB, IKBKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, Serine/threonine protein kinase IKBKB, IKK-2, IKK2, NFKBIKB
- IP
Lab
Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (AB178870)
This immunoprecipation image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/12 dilution. Western blot detection was performed using competitor antibody at 1/100 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (ab178870)
Predicted band size: 87 kDa
Observed band size: 75 kDa,87 kDa
false
- IP
Supplier Data
Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (AB178870)
Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (ab178870)
Predicted band size: 87 kDa
Observed band size: 75-87 kDa
false
- IP
Supplier Data
Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (AB178870)
Cross-Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab32041 (IKK alpha) at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (ab178870)
Predicted band size: 87 kDa
Observed band size: 75 kDa
false
- WB
Lab
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer : 5% NFDM/TBST.
All lanes:
Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution
All lanes:
Human fetal kidney lysates
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
false
- WB
Supplier Data
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
Blocking/dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
All lanes:
Human fetal kidney lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
false
- WB
Lab
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
Western blot : Anti-IKKa/b antibody [EPR16628] (ab178870) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab178870 was shown to bind specifically to IKKa/b. A band was observed at 85 kDa in wild-type HeLa cell lysates with no signal observed at this size in IKKa/b knockout cell line. To generate this image, wild-type and IKKa/b knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human IKBKB (IKK beta) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ikbkb-ikk-beta-knockout-hela-cell-line-ab264847'>ab264847</a>) at 20 µg
Lane 2:
IKKa/b knockout HeLa cell lysate at 20 µg
false
- WB
Supplier Data
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
Blocking/dilution buffer : 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Western blot - Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
Lane 2:
Daudi (Human Burkitt's lymphoma cell line) whole cell lysates at 20 µg
Lane 3:
HL-60 (Human promyelocytic leukemia cells) whole cell lysates at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 75 kDa,87 kDa
false
- WB
Lab
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
This western blot image is a comparison of ab178870 against a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer : 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution
Lane 1:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2:
Daudi (Human Burkitt's lymphoma cell line) whole cell lysates
Lane 3:
HL-60 (Human promyelocytic leukemia cells) whole cell lysates
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 75 kDa,87 kDa
false
- WB
Supplier Data
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
Blocking/dilution buffer : 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Western blot - Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1:
A431 (Human epidermoid carcinoma) whole cell lysates at 10 µg
Lane 2:
293T (Human epithelial cells from embryonic kidney) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 75 kDa,87 kDa
false
- WB
Lab
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer : 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200
Lane 1:
A431 (Human epidermoid carcinoma) whole cell lysates
Lane 2:
293T (Human epithelial cells from embryonic kidney) whole cell lysates
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 75 kDa,87 kDa
false
- WB
Supplier Data
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
Blocking/dilution buffer : 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Western blot - Anti- IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution
Lane 1:
Mouse brain lysates at 10 µg
Lane 2:
Mouse kidney lysates at 10 µg
Lane 3:
Mouse spleen lysates at 10 µg
Lane 4:
Rat kidney lysates at 10 µg
Lane 5:
C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 6:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg
Lane 7:
NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 75 kDa,87 kDa
false
- WB
Lab
Western blot - Anti- IKK beta antibody [EPR16628] (AB178870)
This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.
Blocking/dilution buffer : 5% NFDM/TBST.
ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.
All lanes:
Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution
Lane 1:
Mouse brain lysates
Lane 2:
Mouse kidney lysates
Lane 3:
Mouse spleen lysates
Lane 4:
Rat kidney lysates
Lane 5:
C6 (Rat glial tumor cells) whole cell lysates
Lane 6:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 7:
NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 75 kDa,87 kDa
false
Related conjugates and formulations (1)
-
Anti- IKK beta antibody [EPR16628] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (70)
Recent publications for all applications. Explore the full list and refine your search
Journal of translational medicine 23:632 PubMed40481471
2025
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410438 PubMed39985225
2025
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Frontiers in pharmacology 15:1539363 PubMed39845801
2025
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Scientific reports 14:23989 PubMed39402264
2024
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Cell transplantation 33:9636897241261234 PubMed39068549
2024
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309387 PubMed38889281
2024
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Cell biology international 48:1138-1147 PubMed38769645
2024
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Nature communications 15:438 PubMed38200007
2024
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Biological & pharmaceutical bulletin 47:175-186 PubMed38092386
2023
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Military Medical Research 10:56 PubMed38001521
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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