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Rabbit Recombinant Monoclonal IKK beta antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (AB240210), expandable thumbnail
  • Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (AB240210), expandable thumbnail
  • Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (AB240210), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Not recommended
Not recommended
Mouse
Predicted
Expected
Not recommended
Not recommended
Rat
Predicted
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Rat, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:30337470, PubMed:9346484). Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation (PubMed:9346484). Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis (PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:9346484). In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE (PubMed:11297557, PubMed:14673179, PubMed:20410276, PubMed:21138416). IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs (PubMed:11297557, PubMed:20410276, PubMed:21138416). Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor (PubMed:15084260). Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 (PubMed:17213322, PubMed:19716809). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (PubMed:25326418). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at 'Thr-749' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses (PubMed:38621137). IKBKB-mediated phosphorylation of STAT1 at 'Thr-749' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription (PubMed:32209697).

Alternative names

IKKB, IKBKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, Serine/threonine protein kinase IKBKB, IKK-2, IKK2, NFKBIKB

Recommended products

Rabbit Recombinant Monoclonal IKK beta antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16628
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240210 is the carrier-free version of Anti- IKK beta antibody [EPR16628] ab178870.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (ab240210), expandable thumbnail

    Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (ab240210)

    Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using Anti- IKK beta antibody [EPR16628] ab178870 at 1/40 dilution. Western blot detection was performed using Anti- IKK beta antibody [EPR16628] ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST. 

    Anti- IKK beta antibody [EPR16628] ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti- IKK beta antibody [EPR16628] ab178870).

    All lanes: Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (Anti- IKK beta antibody [EPR16628] ab178870)

    Predicted band size: 87 kDa

    Observed band size: 75-87 kDa

  • Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (ab240210), expandable thumbnail

    Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (ab240210)

    Cross-Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using Anti- IKK beta antibody [EPR16628] ab178870 at 1/40 dilution. Western blot detection was performed using Anti-IKK alpha antibody [Y463] ab32041 (IKK alpha) at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti- IKK beta antibody [EPR16628] ab178870).

    All lanes: Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (Anti- IKK beta antibody [EPR16628] ab178870)

    Predicted band size: 87 kDa

    Observed band size: 75 kDa

  • Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (ab240210), expandable thumbnail

    Immunoprecipitation - Anti- IKK beta antibody [EPR16628] - BSA and Azide free (ab240210)

    This immunoprecipation image is a comparison between Anti- IKK beta antibody [EPR16628] ab178870 and a competitor's leading rabbit polyclonal antibody.

    Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using Anti- IKK beta antibody [EPR16628] ab178870 at 1/40 dilution. Western blot detection was performed using Anti- IKK beta antibody [EPR16628] ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.

    Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using competitor’s Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/12 dilution. Western blot detection was performed using competitor antibody at 1/100 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100 dilution. The blocking and diluting buffer was 5% NFDM/TBST. 

    Anti- IKK beta antibody [EPR16628] ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti- IKK beta antibody [EPR16628] ab178870).

    All lanes: Immunoprecipitation - Anti- IKK beta antibody [EPR16628] (Anti- IKK beta antibody [EPR16628] ab178870)

    Predicted band size: 87 kDa

    Observed band size: 75 kDa, 87 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com