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Rabbit Recombinant Monoclonal IKK gamma/NEMO antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (AB188569), expandable thumbnail
  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (AB188569), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR14660] (AB188569), expandable thumbnail
  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (AB188569), expandable thumbnail
  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (AB188569), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor (PubMed:14695475, PubMed:20724660, PubMed:21518757, PubMed:9751060). Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways (PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:19185524, PubMed:21606507, PubMed:27777308, PubMed:33567255). Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin (PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:19185524, PubMed:21606507, PubMed:27777308). Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3 (PubMed:19854139). Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination (PubMed:20724660). (Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B.

Alternative names

FIP3, NEMO, IKBKG, NF-kappa-B essential modulator, FIP-3, IkB kinase-associated protein 1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, NF-kappa-B essential modifier, IKKAP1, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma

Recommended products

Rabbit Recombinant Monoclonal IKK gamma/NEMO antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14660
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    Lanes 1- 2: Merged signal (red and green). Green - ab188569 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab188569 was shown to react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in knockout cell line Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line ab266674 (knockout cell lysate Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and IKBKG knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188569 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: IKBKG knockout HEK-293T cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: IKK gamma/NEMO knockout HAP1 cell lysate (20 μg)
    Lane 3: Jurkat cell lysate (20 μg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab188569 observed at 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab188569 was shown to react with IKK gamma/NEMO in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IKK gamma/NEMO knockout samples were examined. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. ab188569 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    Immunocytochemical analysis of Jurkat cells fixed in 4% paraformaldehyde labeling IKK gamma/NEMO with ab188569 at 1/100 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI.

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    Lanes 1- 2: Merged signal (red and green). Green - ab188569 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab188569 was shown to react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line ab266674 (CRISPR/Cas9 edited cell lysate Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and IKBKG CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188569 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: IKBKG CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line (Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line ab266674)

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569)

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (ab188569) at 1/10000 dilution

    Lane 1: Hela cell lysate at 20 µg

    Lane 2: Jurkat cell lysate at 20 µg

    Lane 3: K562 cell lysate at 20 µg

    Lane 4: Human fetal brain lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 41 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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