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Rabbit Recombinant Monoclonal IKK gamma/NEMO antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 27 publications.


Images

Immunoprecipitation - Anti-IKK gamma/NEMO antibody [EPR16629] (AB178872), expandable thumbnail
  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (AB178872), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR16629] (AB178872), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK gamma/NEMO antibody [EPR16629] (AB178872), expandable thumbnail
  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (AB178872), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Expected
Rat
Expected
Tested
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/5000

Notes

-

Species

Human

Dilution info

1/5000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/250

Notes

-

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor (PubMed:14695475, PubMed:20724660, PubMed:21518757, PubMed:9751060). Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways (PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:19185524, PubMed:21606507, PubMed:27777308, PubMed:33567255). Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin (PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:19185524, PubMed:21606507, PubMed:27777308). Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3 (PubMed:19854139). Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination (PubMed:20724660).(Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B.

Alternative names

FIP3, NEMO, IKBKG, NF-kappa-B essential modulator, NEMO, FIP-3, IkB kinase-associated protein 1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, NF-kappa-B essential modifier, IKKAP1, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma

Recommended products

Rabbit Recombinant Monoclonal IKK gamma/NEMO antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 27 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16629

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunoprecipitation - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Immunoprecipitation - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    IKK gamma/NEMO was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab178872 at 1/50 dilution. Western blot was performed of the immunoprecipitate using ab178872 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: HeLa whole cell extract. Right lane: PBS instead of HeLa whole cell extract.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Predicted band size: 48 kDa

    Observed band size: 37-60 kDa

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: IKK gamma/NEMO knockout HAP1 cell lysate (20 μg)
    Lane 3: Jurkat cell lysate (20 μg)
    Lane 4: K562 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab178872 observed at 40, 45, 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab178872 was shown to react with IKK gamma in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IKK gamma/NEMO knockout samples were examined. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. ab178872 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Immunofluorescence analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells (Mouse embyro fibroblast cells) labeling IKK gamma/NEMO (green) with ab178872 at 1/250 dilution showing cytoplasm and nucleus staining. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution with secondary antibody Goat anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178872 at 1/250 dilution, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/400 dilution. -ve control 2 is Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Immunohistochemical analysis of paraffin embedded human colonic adenocarcinoma tissue labeling IKK gamma/NEMO with ab178872 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on colonic adenocarcinoma is observed.

    Negative control: Using PBS instead of primary ab, secondary ab ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Lanes 1- 2: Merged signal (red and green). Green - ab178872 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab178872 was shown to react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in knockout cell line Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line ab266674 (knockout cell lysate Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and IKBKG knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178872 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: IKBKG knockout HEK-293T cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    ab178872 could recognize 3 isoforms with the predicted MWs of 37KDa, 56KDa and 48KDa, respectively.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872) at 1/5000 dilution

    Lane 1: Human fetal brain at 10 µg

    Lane 2: Human fetal kidney at 10 µg

    Lane 3: Human colon cancer at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 37 kDa, 48 kDa, 56 kDa

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    ab178872 could recognize 3 isoforms with the predicted MWs of 37KDa, 56KDa and 48KDa, respectively.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872) at 1/20000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

    Lane 2: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 46-60 kDa

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Lanes 1- 2: Merged signal (red and green). Green - ab178872 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab178872 was shown to react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line ab266674 (CRISPR/Cas9 edited cell lysate Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and IKBKG CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178872 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: IKBKG CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872) at 1/20000 dilution

    Lane 1: Mouse brain at 10 µg

    Lane 2: Mouse heart at 10 µg

    Lane 3: Mouse kidney at 10 µg

    Lane 4: Mouse spleen at 10 µg

    Lane 5: Rat brain at 10 µg

    Lane 6: Rat heart at 10 µg

    Lane 7: Rat kidney at 10 µg

    Lane 8: Rat spleen at 10 µg

    Lane 9: C6 (Rat glial tumor cells) whole cell lysates at 10 µg

    Lane 10: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

    Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

    Lane 12: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 37-60 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Immunofluorescence analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling IKK gamma/NEMO (green) with ab178872 at 1/250 dilution showing cytoplasm and nucleus staining. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution with secondary antibody Goat anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178872 at 1/250 dilution, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/400 dilution. -ve control 2 is Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK gamma/NEMO antibody [EPR16629] (ab178872)

    Immunohistochemical analysis of paraffin embedded Rat colon tissue labeling IKK gamma/NEMO with ab178872 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stain is Hematoxylin. Cytoplasm staining on epithelial cells of rat colon is observed.

    Negative control: Using PBS instead of primary ab, secondary ab as above.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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