Anti-IKKi/IKKe antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (AB7891)

ab7891 at 10μg/ml staining IKK epsilon in human pancreatic tissue by IHC.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (AB7891)

Immunocytochemistry/Immunofluorecense analysis of HeLa cells with ab7891 at 20μg/ml.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (AB7891)

ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• WB

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Western blot - Anti-IKKi/IKKe antibody (AB7891)

All lanes:

Western blot - Anti-IKKi/IKKe antibody (ab7891) at 1 µg/mL

All lanes:

Jurkat whole cell lysate

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-IKKi/IKKe antibody (AB7891)

All lanes:

Western blot - Anti-IKKi/IKKe antibody (ab7891) at 1 µg/mL

Lane 1:

A431 cell lysate at 15 µg

Lane 2:

Daudi cell lysate at 15 µg

Lane 3:

Jurkat cell lysate at 15 µg

Lane 4:

SK-N-SH cell lysate at 15 µg

Lane 5:

3T3/NIH cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-IKKi/IKKe antibody (AB7891)

All lanes:

Western blot - Anti-IKKi/IKKe antibody (ab7891) at 1 µg/mL

Lane 1:

Untreated Raw264.7 cell lysates

Lane 2:

Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 3 hrs

Lane 3:

Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 6 hrs

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 80 kDa

false