Rabbit Polyclonal IKKi/IKKe antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 17 publications.
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- Journal of virology 96:e01125222022Substitution of S179P in the Lyssavirus Phosphoprotein Impairs Its Interferon Antagonistic Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZongmei Wang,Yueming Yuan,Yuan Zhang,Chengguang Zhang,Baokun Sui,Jianqing Zhao,Ming Zhou,Huanchun Chen,Zhen F Fu,Ling ZhaoPubMed 36326274
- Journal of translational medicine 19:3962021CYT387, a potent IKBKE inhibitor, suppresses human glioblastoma progression by activating the Hippo pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wang,Jie Lu,Jing Li,Yang Liu,Gaochao Guo,Qiang HuangPubMed 34544426
- Aging 13:14304-143212021Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Wu,Xinyi Zheng,Zhixian Yao,Zhong Zheng,Wenjie Huang,Xingyu Mu,Feng Sun,Zhihong Liu,Junhua ZhengPubMed 34016791
- Viruses 13:2021Potential Dual Role of West Nile Virus NS2B in Orchestrating NS3 Enzymatic Activity in Viral Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlanna C Tseng,Vivek R Nerurkar,Kabi R Neupane,Helmut Kae,Pakieli H KaufusiPubMed 33572517
- Biochemical pharmacology 180:1141262020Macrophages confer resistance to BET inhibition in triple-negative breast cancer by upregulating IKBKE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianghua Qiao et. al.PubMed 32603665
- Theranostics 10:2358-23732020IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGe Liu et. al.PubMed 32104508
- Cancer management and research 11:6593-66022019Expression and prognostic role of IKBKE and TBK1 in stage I non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wang et. al.PubMed 31406474
- Biochimica et biophysica acta. Molecular basis of 1865:1421-14272019Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Yuan et. al.PubMed 30794866
- BMC cancer 18:5952018IΚΚε cooperates with either MEK or non-canonical NF-kB driving growth of triple-negative breast cancer cells in different contexts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarrie D House et. al.PubMed 29801480
- Frontiers in cell and developmental biology 6:402018Importance of Validating Antibodies and Small Compound Inhibitors Using Genetic Knockout Studies-T Cell Receptor-Induced CYLD Phosphorylation by IKKε/TBK1 as a Case Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Lork et. al.PubMed 29755980
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.
Alternative names
IKKE, IKKI, KIAA0151, IKBKE, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, Inducible I kappa-B kinase, IKK-i
Recommended products
Rabbit Polyclonal IKKi/IKKe antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 17 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification
- Specificity
Recognises IKK epsilon and does not cross react with IKK alpha, beta or gamma.
- Concentration
- Purification notes
Purified IgG was prepared by affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-IKKi/IKKe antibody (ab7891)
All lanes: Western blot - Anti-IKKi/IKKe antibody (ab7891) at 1 µg/mL
Lane 1: Untreated Raw264.7 cell lysates
Lane 2: Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 3 hrs
Lane 3: Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 6 hrs
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa
Western blot - Anti-IKKi/IKKe antibody (ab7891)
All lanes: Western blot - Anti-IKKi/IKKe antibody (ab7891) at 1 µg/mL
Lane 1: A431 cell lysate at 15 µg
Lane 2: Daudi cell lysate at 15 µg
Lane 3: Jurkat cell lysate at 15 µg
Lane 4: SK-N-SH cell lysate at 15 µg
Lane 5: 3T3/NIH cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa
Western blot - Anti-IKKi/IKKe antibody (ab7891)
All lanes: Western blot - Anti-IKKi/IKKe antibody (ab7891) at 1 µg/mL
All lanes: Jurkat whole cell lysate
Predicted band size: 80 kDa
Observed band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)
ab7891 at 10μg/ml staining IKK epsilon in human pancreatic tissue by IHC.
Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)
ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)
Immunocytochemistry/Immunofluorecense analysis of HeLa cells with ab7891 at 20μg/ml.
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