AB139182

Anti-IKZF3 antibody [EPR9343(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal IKZF3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ZNFN1A3, IKZF3, Zinc finger protein Aiolos, Ikaros family zinc finger protein 3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody [EPR9343(B)] (AB139182)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody [EPR9343(B)] (AB139182)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling IKZF3 with ab139182 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-IKZF3 antibody [EPR9343(B)] (AB139182)

All lanes:

Western blot - Anti-IKZF3 antibody [EPR9343(B)] (ab139182) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa

false

Carrier free

Anti-IKZF3 antibody [EPR9343(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9343(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.

See full target information IKZF3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 22:1440-1451 PubMed34686860

2021

Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathology.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Fujiwara,Keisuke Seike,Michael D Brooks,Anna V Mathew,Ilya Kovalenko,Anupama Pal,Ho-Joon Lee,Daniel Peltier,Stephanie Kim,Chen Liu,Katherine Oravecz-Wilson,Lu Li,Yaping Sun,Jaeman Byun,Yoshinobu Maeda,Max S Wicha,Thomas L Saunders,Alnawaz Rehemtulla,Costas A Lyssiotis,Subramaniam Pennathur,Pavan Reddy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IKZF3 antibody [EPR9343(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody [EPR9343(B)] (AB139182)

Western blot - Anti-IKZF3 antibody [EPR9343(B)] (AB139182)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (1)

Product promise