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Mouse Recombinant Monoclonal IKZF3 antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples.

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Images

Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (AB269478), expandable thumbnail
  • ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (AB269478), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCELISAWB
Human
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.

Alternative names

ZNFN1A3, IKZF3, Zinc finger protein Aiolos, Ikaros family zinc finger protein 3

Recommended products

Mouse Recombinant Monoclonal IKZF3 antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
1C3
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab269478 is the carrier-free version of Anti-IKZF3 Degron tag antibody [1C3] ab269464.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478), expandable thumbnail

    Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

    This data was produced using Anti-IKZF3 Degron tag antibody [1C3] ab269464, the same antibody clone but in a different formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-IKZF3 Degron tag antibody [1C3] (Anti-IKZF3 Degron tag antibody [1C3] ab269464) at 1/1000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-mCherry, whole cell lysate at 20 µg

    Lane 2: HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Exposure time: 10s

  • ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478), expandable thumbnail

    ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

    This data was produced using Anti-IKZF3 Degron tag antibody [1C3] ab269464, the same antibody clone but in a different formulation.

    Anti-IKZF3 Degron tag antibody [1C3] ab269464 used at 0-4000 ng/ml.

    Antigen (Human IKZF3 Degron tag) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com