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AB269478

Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free

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Mouse Recombinant Monoclonal IKZF3 antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples.

View Alternative Names

ZNFN1A3, IKZF3, Zinc finger protein Aiolos, Ikaros family zinc finger protein 3

2 Images
ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (AB269478)
  • ELISA

Lab

ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (AB269478)

This data was produced using ab269464, the same antibody clone but in a different formulation.

ab269464 used at 0-4000 ng/ml.

Antigen (Human IKZF3 Degron tag) used at 1000 ng/ml.

Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (AB269478)
  • WB

Lab

Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (AB269478)

This data was produced using ab269464, the same antibody clone but in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IKZF3 Degron tag antibody [1C3] (<a href='/en-us/products/primary-antibodies/ikzf3-degron-tag-antibody-1c3-ab269464'>ab269464</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-mCherry, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 10s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C3

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB191394

Anti-Ikaros antibody [EPR13790]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ikaros antibody [EPR13790] (AB191394)

  • 0
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Primary Antibodies

AB313814

Anti-ZNFN1A2/HELIOS antibody [EPR28015-91] - BSA and Azide free (Detector)

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Sandwich ELISA - Anti-ZNFN1A2/HELIOS antibody [EPR28015-91] - BSA and Azide free (Detector) (AB313814)

  • 0
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Primary Antibodies

AB273625

Anti-IL-15 antibody [EPR22635-214]

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Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625)

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Primary Antibodies

AB30394

Anti-Integrin beta 1 antibody [12G10]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [12G10] (AB30394)

  • 5
  • 9
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab269478 is the carrier-free version of ab269464.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
See full target information IKZF3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com