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AB220329

Anti-IKZF4 antibody

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(3 Publications)

Rabbit Polyclonal IKZF4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IKZF4 aa 300-450.

View Alternative Names

KIAA1782, ZNFN1A4, IKZF4, Zinc finger protein Eos, Ikaros family zinc finger protein 4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-IKZF4 antibody (AB220329)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IKZF4 antibody (AB220329)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling IKZF4 with ab220329 at 4 μg/ml. Antibody staining is shown in green.

Western blot - Anti-IKZF4 antibody (AB220329)
  • WB

Supplier Data

Western blot - Anti-IKZF4 antibody (AB220329)

All lanes:

Western blot - Anti-IKZF4 antibody (ab220329) at 1/100 dilution

All lanes:

RT4 cell extract

Predicted band size: 64 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IKZF4 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q9H2S9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (By similarity).
See full target information IKZF4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 41:726-739.e11 PubMed36898380

2023

Dual IKZF2 and CK1α degrader targets acute myeloid leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sun-Mi Park,David K Miyamoto,Grace Y Q Han,Mandy Chan,Nicole M Curnutt,Nathan L Tran,Anthony Velleca,Jun Hyun Kim,Alexandra Schurer,Kathryn Chang,Wenqing Xu,Michael G Kharas,Christina M Woo

Virology journal 16:160 PubMed31856855

2019

EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.

Applications

Unspecified application

Species

Unspecified reactive species

Tadasuke Naito,Hiroshi Ushirogawa,Takuya Fukushima,Yuetsu Tanaka,Mineki Saito

Cell cycle (Georgetown, Tex.) 19:15-23 PubMed31760894

2019

SET8 localization to chromatin flanking DNA damage is dependent on RNF168 ubiquitin ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Stanimir Dulev,Sichun Lin,Qingquan Liu,Vildan B Cetintas,Nizar N Batada
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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