Rabbit Polyclonal IL-1 alpha antibody. Suitable for IHC-P, ELISA, WB, sELISA, Neut, ICC/IF and reacts with Human, Recombinant full length protein, Recombinant full length protein - Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL1A.
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Publications
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- Fluids and barriers of the CNS 19:782022The effect of A1 and A2 reactive astrocyte expression on hydrocephalus shunt failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Khodadadei,Rooshan Arshad,Diego M Morales,Jacob Gluski,Neena I Marupudi,James P McAllister,David D Limbrick,Carolyn A HarrisPubMed 36171630
- Head and neck pathology 16:763-7722022Comparison of Interleukin-1 Ligand Expression by Human Papilloma Virus Status in HNSCCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIshrat Nourin Khan,Katherine N Gibson-Corley,Joseph D Coppock,Andrean L SimonsPubMed 35334093
- Circulation 144:893-9082021Interleukin-1α Is a Central Regulator of Leukocyte-Endothelial Adhesion in Myocardial Infarction and in Chronic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan J Schunk,Sarah Triem,David Schmit,Stephen Zewinger,Tamim Sarakpi,Ellen Becker,Gregor Hütter,Selina Wrublewsky,Fabienne Küting,Mathias Hohl,Dalia Alansary,Leticia Prates Roma,Peter Lipp,Julia Möllmann,Michael Lehrke,Matthias W Laschke,Michael D Menger,Rafael Kramann,Peter Boor,Willi Jahnen-Dechent,Winfried März,Michael Böhm,Ulrich Laufs,Barbara A Niemeyer,Danilo Fliser,Emmanuel Ampofo,Thimoteus SpeerPubMed 34192892
- Aging cell 19:e131982020CD8 T cells are increased in the subventricular zone with physiological and pathological aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel Moreno-Valladares et. al.PubMed 32741087
- Cell reports 31:1077762020Neurotoxic Reactive Astrocytes Drive Neuronal Death after Retinal Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin A Guttenplan,Benjamin K Stafford,Rana N El-Danaf,Drew I Adler,Alexandra E Münch,Maya K Weigel,Andrew D Huberman,Shane A LiddelowPubMed 32579912
- Antioxidants (Basel, Switzerland) 9:2020Baicalein Inhibits Benzo[a]pyrene-Induced Toxic Response by Downregulating Src Phosphorylation and by Upregulating NRF2-HMOX1 System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuka Tanaka et. al.PubMed 32526964
- Cancer management and research 11:10623-106322019High Inflammatory Factor Grading Predicts Poor Disease-Free Survival in AJCC Stage I-II Hepatocellular Carcinoma Patients After R0 Resection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMao Zhang et. al.PubMed 31908534
- Journal of oncology 2019:58596802019Impact of Nuclear Interleukin-1 Alpha and EGFR Expression on Recurrence and Survival Outcomes in Oral Squamous Cell Carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnand Rajan et. al.PubMed 31320902
- Molecular and cellular biology 39:2019Uncoupling the Senescence-Associated Secretory Phenotype from Cell Cycle Exit via Interleukin-1 Inactivation Unveils Its Protumorigenic Role.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLena Lau et. al.PubMed 30988157
- Glia 67:802-8242018Human immunodeficiency virus Type-1 single-stranded RNA activates the NLRP3 inflammasome and impairs autophagic clearance of damaged mitochondria in human microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPratima Rawat et. al.PubMed 30582668
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IL1A. Database link P01583
Consider this alternative
Reactivity data
Select an application| IHC-P | ELISA | WB | sELISA | Neut | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Predicted | Expected | Expected | Expected | Expected |
| Recombinant full length protein | Not recommended | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes To detect hIL-1a by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271226 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 μg/ml of this antibody is required. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed:26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed:17507369, PubMed:2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed:17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed:14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed:15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed:26439902).
Alternative names
IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1
Recommended products
Rabbit Polyclonal IL-1 alpha antibody. Suitable for IHC-P, ELISA, WB, sELISA, Neut, ICC/IF and reacts with Human, Recombinant full length protein, Recombinant full length protein - Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL1A.
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IL1A. Database link P01583
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-IL-1 alpha antibody ab7632
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody (ab9614)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IL-1 alpha with ab9614 at 1 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Western blot - Anti-IL-1 alpha antibody (ab9614)
All lanes: Western blot - Anti-IL-1 alpha antibody (ab9614) at 1 µg/mL
All lanes: Recombinant human IL-1 alpha protein (ab73567) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Exposure time: 30s
Sandwich ELISA - Anti-IL-1 alpha antibody (ab9614)
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