AB206410

Anti-IL-1 alpha antibody [EPR19904-247]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal IL-1 alpha antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

3 Images

Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green)labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Blocking/Dilution buffer : 5% NFDM/TBST.

Expression of IL-1 alpha can be induced by LPS in macrophages. The molecular masses observed are consistent with the literature (PMID : 9785472; PMID : 1868253; PMID : 19104081).

All lanes:

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

THP-1, treated with 50 nM phorbol-12-myristate-13-acetate (PMA, <a href='/en-us/products/biochemicals/phorbol-12-myristate-13-acetate-pma-pkc-activator-ab120297'>ab120297</a>) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 31 kDa

Observed band size: 15 kDa,20 kDa,34 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

All lanes:

Human IL-1 alpha recombinant protein (aa113-271) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

false

Exposure time: 3s

Carrier free

Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19904-247

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed : 17507369, PubMed : 2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed : 17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed : 15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed : 26439902).

See full target information IL1A

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiothoracic surgery 19:314 PubMed38824534

2024

Long non-coding RNA MEG3 knockdown represses airway smooth muscle cells proliferation and migration via sponging miR-143-3p/FGF9 in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Gu,Dengfeng Zhou

Asian Pacific journal of cancer prevention : APJCP 23:3843-3849 PubMed36444597

2022

A Comparison of the Characteristics of Cytokine Storm between Lichen Planus and Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Salmeh Kalbassi,Lida Radfar,Mohammadreza Azimi,Simin Shadanpoor,Ali Ghorbani Ranjbary

NPJ breast cancer 8:30 PubMed35260569

2022

Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew W Chung,Anthony J Kozielski,Wei Qian,Jianying Zhou,Ann C Anselme,Alfred A Chan,Ping-Ying Pan,Delphine J Lee,Jenny C Chang

Frontiers in pharmacology 12:673818 PubMed34093202

2021

An 8-Hydroxy-Quinoline Derivative Protects Against Lipopolysaccharide-Induced Lethality in Endotoxemia by Inhibiting HMGB1-Mediated Caspase-11 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Wang,Jian Shi,Zhaozheng Li,Ling Li,Rui Zhang,Yang Bai,Junmei Li,Fang Liang,Yiting Tang

Cell death & disease 12:402 PubMed33854044

2021

A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Wang,Zhaozheng Li,Yang Bai,Rui Zhang,Ran Meng,Fangping Chen,Haichao Wang,Timothy R Billiar,Xianzhong Xiao,Ben Lu,Yiting Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-1 alpha antibody [EPR19904-247]

Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

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