Skip to main content

Rabbit Recombinant Monoclonal IL-1 alpha antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Anti-IL-1 alpha antibody [EPR20715-16] - BSA and Azide free (Capture) (AB244774), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Rat
Expected

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.

Alternative names

Interleukin-1 alpha, IL-1 alpha, Il1a

Recommended products

Rabbit Recombinant Monoclonal IL-1 alpha antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR20715-16
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab244774 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat IL-1 alpha. The recommended pair for sandwich ELISA is:
Capture: ab244774, Rat IL-1 alpha Capture Antibody (unconjugated)
Detector: Anti-IL-1 alpha antibody [EPR20715-82] - BSA and Azide free (Detector) ab245047, Rat IL-1 alpha Detector Antibody (unconjugated)
The reference range value is 5.47 - 350 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-IL-1 alpha antibody [EPR20715-16] - BSA and Azide free (Capture) (ab244774), expandable thumbnail

    Sandwich ELISA - Anti-IL-1 alpha antibody [EPR20715-16] - BSA and Azide free (Capture) (ab244774)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Rat IL-1 alpha ELISA Kit ab235646).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com