Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (AB300502)

This data was developed using ab300501, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IL-1 alpha with ab300501 at 1/5000 (0.106 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on immune cells of human colon. The section was incubated with ab300501 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (AB300502)

This data was developed using ab300501, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling IL-1 alpha with ab300501 at 1/5000 (0.106 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on immune cells of human colon cancer. The section was incubated with ab300501 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (AB300502)

This data was developed using ab300501, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded A Mouse lung treate tissue labeling IL-1 alpha with ab300501 at 1/5000 (0.106 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse lung induced by LPS (A) and no staining on untreated mouse lung. The section was incubated with ab300501 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (AB300502)

This data was developed using ab300501, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling IL-1 alpha with ab300501 at 1/50 (10.62 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing increased nuclear staining in Raw 264.7 cells treated with lipopolysaccharide (10 ug/ml) for 24 h (PMID : 25870118) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

• WB

Supplier Data

Western blot - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (AB300502)

This data was developed using ab300501, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-1 alpha antibody [EPR25263-3] (<a href='/en-us/products/primary-antibodies/il-1-alpha-antibody-epr25263-3-ab300501'>ab300501</a>) at 1/1000 dilution

Lane 1:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lane 2:

RAW264.7 treated with /ml lipopolysaccharide (LPS) for 7 hours, whole cell lysate 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 31 kDa

false

Exposure time: 15s