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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal IL-1 beta antibody. Suitable for IHC-P, WB, sELISA and reacts with Human, Recombinant full length protein - Mouse samples. Cited in 865 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Il1b aa 100 to C-terminus.
Unknown
Rabbit
Standard buffer
Lyophilized
Polyclonal
IHC-P | WB | sELISA | |
---|---|---|---|
Human | Tested | Expected | Expected |
Recombinant full length protein - Mouse | Not recommended | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Mouse | Dilution info 0.1-0.2 µg/mL | Notes Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Mouse | Dilution info 2 µg/mL | Notes To detect mIL-1b by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819).
Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
Rabbit Polyclonal IL-1 beta antibody. Suitable for IHC-P, WB, sELISA and reacts with Human, Recombinant full length protein - Mouse samples. Cited in 865 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Il1b aa 100 to C-terminus.
Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
Unknown
Rabbit
Standard buffer
Lyophilized
Polyclonal
Affinity purification Immunogen
Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta.
unknown
Reconstitute with sterile water to 0.1-1.0mg/ml, aliquot and store at -20°C.
Blue Ice
1-2 weeks
+4°C
-20°C
Avoid freeze / thaw cycle
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab156791
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal adrenal tissue labelling IL-1 beta with ab9722 at 2 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA detecting IL-1 beta using ab9722 at a concentration of 2 μg/ml.
To detect mouse IL-1 beta by Western Blot analysis, ab9722 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse IL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse IL-1 beta, respectively.
Non-reducing conditions.
All lanes: Western blot - Anti-IL-1 beta antibody (AB9722)
Predicted band size: 30 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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