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Rabbit Polyclonal IL-1 beta antibody. Suitable for IHC-P, WB, sELISA and reacts with Human, Recombinant full length protein - Mouse samples. Cited in 865 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Il1b aa 100 to C-terminus.


Images

Key facts

Isotype

Unknown

Host species

Rabbit

Storage buffer

Standard buffer

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Full Length Protein corresponding to Mouse Il1b aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10749

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBsELISA
Human
Tested
Expected
Expected
Recombinant full length protein - Mouse
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

2 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Mouse

Dilution info

0.1-0.2 µg/mL

Notes

Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Recombinant full length protein - Mouse

Dilution info

2 µg/mL

Notes

To detect mIL-1b by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819).

Alternative names

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Rabbit Polyclonal IL-1 beta antibody. Suitable for IHC-P, WB, sELISA and reacts with Human, Recombinant full length protein - Mouse samples. Cited in 865 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Il1b aa 100 to C-terminus.

Alternative names

Key facts

Isotype

Unknown

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Full Length Protein corresponding to Mouse Il1b aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10749
Purification technique

Affinity purification Immunogen

Specificity

Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta.

Light chain type

unknown

Reconstitution

Reconstitute with sterile water to 0.1-1.0mg/ml, aliquot and store at -20°C.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab156791

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal adrenal tissue labelling IL-1 beta with ab9722 at 2 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • Sandwich ELISA - Anti-IL-1 beta antibody (ab9722), expandable thumbnail

    Sandwich ELISA - Anti-IL-1 beta antibody (ab9722)

    Sandwich ELISA detecting IL-1 beta using ab9722 at a concentration of 2 μg/ml.

  • Western blot - Anti-IL-1 beta antibody (ab9722), expandable thumbnail

    Western blot - Anti-IL-1 beta antibody (ab9722)

    To detect mouse IL-1 beta by Western Blot analysis, ab9722 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse IL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

    Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse IL-1 beta, respectively.

    Non-reducing conditions.

    All lanes: Western blot - Anti-IL-1 beta antibody (AB9722)

    Predicted band size: 30 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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