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Rabbit Polyclonal IL-1 beta antibody. Suitable for sELISA and reacts with Recombinant full length protein - Rat samples. Cited in 87 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Il1b.


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Sandwich ELISA - Anti-IL-1 beta antibody (AB9787), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Standard buffer

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Rat Il1b. Database link Q63264

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Recombinant full length protein - Rat
Tested

Tested
Tested

Species

Recombinant full length protein - Rat

Dilution info

0.5-2 µg/mL

Notes

To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a ab245863 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.

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Target data

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

Alternative names

Recommended products

Rabbit Polyclonal IL-1 beta antibody. Suitable for sELISA and reacts with Recombinant full length protein - Rat samples. Cited in 87 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Il1b.

Key facts

Isotype

IgG

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Rat Il1b. Database link Q63264
Purification technique

Affinity purification Immunogen

Specificity

This antibody is used in our Rat IL-1beta EDK and was tested against the following rat growth factors at 50ng/ml to determine if there was any cross reactivity: GM-CSF, IL1alpha, IL-2, IL-4, IL-10, SCF, & TNFalpha. No significant cross reactivity was detected.
The antibody has not been tested against endogenous IL1beta, only recombinant protein and we cannot guarantee it will detect endogenous protein.

Light chain type

unknown

Reconstitution

Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-IL-1 beta antibody [OTI3E1] ab156791

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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1 product image

  • Sandwich ELISA - Anti-IL-1 beta antibody (ab9787), expandable thumbnail

    Sandwich ELISA - Anti-IL-1 beta antibody (ab9787)

    This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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