Rabbit Polyclonal IL-1 beta antibody. Suitable for sELISA and reacts with Recombinant full length protein - Rat samples. Cited in 87 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Il1b.
IgG
Rabbit
Standard buffer
Lyophilized
Polyclonal
sELISA | |
---|---|
Recombinant full length protein - Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Rat | Dilution info 0.5-2 µg/mL | Notes To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a ab245863 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta. |
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Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Interleukin-1 beta, IL-1 beta, Il1b
Rabbit Polyclonal IL-1 beta antibody. Suitable for sELISA and reacts with Recombinant full length protein - Rat samples. Cited in 87 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Il1b.
IgG
Rabbit
Standard buffer
Lyophilized
Polyclonal
Affinity purification Immunogen
This antibody is used in our Rat IL-1beta EDK and was tested against the following rat growth factors at 50ng/ml to determine if there was any cross reactivity: GM-CSF, IL1alpha, IL-2, IL-4, IL-10, SCF, & TNFalpha. No significant cross reactivity was detected.
The antibody has not been tested against endogenous IL1beta, only recombinant protein and we cannot guarantee it will detect endogenous protein.
unknown
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-IL-1 beta antibody [OTI3E1] ab156791
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This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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