Skip to main content

Rabbit Recombinant Monoclonal IL-1 beta antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 68 publications.


Images

Western blot - Anti-IL-1 beta antibody [EPR16805-15] (AB234437), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] (AB234437), expandable thumbnail
  • Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] (AB234437), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] (AB234437), expandable thumbnail
  • Western blot - Anti-IL-1 beta antibody [EPR16805-15] (AB234437), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Mouse
Tested
Tested
Not recommended
Tested
Tested
Rat
Expected
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/60
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

Alternative names

Interleukin-1 beta, IL-1 beta, Il1b

Recommended products

Rabbit Recombinant Monoclonal IL-1 beta antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 68 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16805-15
Purification technique
Affinity purification Protein A
Specificity

IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437), expandable thumbnail

    Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8446594).

    IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).

    All lanes: Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437) at 1/1000 dilution

    Lane 1: Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa

    Observed band size: 17 kDa, 28 kDa, 31 kDa

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] (ab234437), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilised J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) cells labeling IL-1 beta with ab234437 at 1/50 (10μg/ml) dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used as a secondary antibody at 1/1000 (2μg/ml) dilution. Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594). Nuclear staining: DAPI.

    Confocal image showing cytoplasmic staining in J774A.1 cells treated with Lipopolysaccharides (100 ng/ml) for 4h, with addition of brefeldin A (1 μg/ml) for the last 3 hours.

  • Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] (ab234437), expandable thumbnail

    Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)

    IL-1 beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate with ab234437 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234437 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate 10 μg (Input).
    Lane 2: ab234437 IP in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab234437 in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.

    IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)

    Predicted band size: 30 kDa

    Observed band size: 31 kDa

  • Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] (ab234437), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)

    Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells that were either treated (100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h labeling) (red) or untreated (green) labeling IL-1 beta with ab234437 at 1/60 dilution compared with a rabbit monoclonal IgG Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).

  • Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437), expandable thumbnail

    Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)

    Blocking and dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437) at 1/1000 dilution

    Lanes 1 and 3: Untreated NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg

    Lane 2: NR8383 (rat lung macrophage (alveolar)) treated with 100ng/ml IFN gamma and 10ng TNF alpha for 32 hours, then with 5 µg/ml Brefeldin A (BFA) added for 6 hours whole cell lysate at 20 µg

    Lane 4: NR8383 (rat lung macrophage (alveolar)) treated with 100ng/ml LPS and 300ng /ml Brefeldin A (BFA) for 6 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20 kDa, 31 kDa

    Exposure time: 40s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com