Rabbit Recombinant Monoclonal IL-1 beta antibody. Carrier free. Suitable for WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--immunocytochemistry-immunofluorescence-img175737.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195)")
![Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--immunoprecipitation-img4098.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195)")
![Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--flow-cytometry-intracellular-img25344.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195)")
![Western blot - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--western-blot-img423810.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (AB254195)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Mouse | Expected | Tested | Not recommended | Tested | Tested |
| Rat | Tested | Expected | Not recommended | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
Recommended products
Rabbit Recombinant Monoclonal IL-1 beta antibody. Carrier free. Suitable for WB, IP, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16805-15
- Purification technique
- Affinity purification Protein A
- Specificity
IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab254195 is the carrier-free version of Anti-IL-1 beta antibody [EPR16805-15] ab234437.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--immunocytochemistry-immunofluorescence-img175737.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-1 beta antibody [EPR16805-15] ab234437).
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilised J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) cells labeling IL-1 beta with Anti-IL-1 beta antibody [EPR16805-15] ab234437 at 1/50 (10μg/ml) dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used as a secondary antibody at 1/1000 (2μg/ml) dilution. Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594). Nuclear staining: DAPI.
Confocal image showing cytoplasmic staining in J774A.1 cells treated with Lipopolysaccharides (100 ng/ml) for 4h, with addition of brefeldin A (1 μg/ml) for the last 3 hours.
![Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--immunoprecipitation-img4098.jpg "Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)")
Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)
IL-1 beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate with Anti-IL-1 beta antibody [EPR16805-15] ab234437 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-IL-1 beta antibody [EPR16805-15] ab234437 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate 10 μg (Input).
Lane 2: Anti-IL-1 beta antibody [EPR16805-15] ab234437 IP in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL-1 beta antibody [EPR16805-15] ab234437 in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-1 beta antibody [EPR16805-15] ab234437).
All lanes: Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] (Anti-IL-1 beta antibody [EPR16805-15] ab234437)
Predicted band size: 30 kDa
Observed band size: 31 kDa
![Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--flow-cytometry-intracellular-img25344.jpg "Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)")
Flow Cytometry (Intracellular) - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)
Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells that were either treated (100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h labeling) (red) or untreated (green) labeling IL-1 beta with Anti-IL-1 beta antibody [EPR16805-15] ab234437 at 1/60 dilution compared with a rabbit monoclonal IgG Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-1 beta antibody [EPR16805-15] ab234437).
![Western blot - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr16805-15-bsa-and-azide-free-ab254195--western-blot-img423810.jpg "Western blot - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)")
Western blot - Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (ab254195)
This data was developed using the same antibody clone in a different buffer formulation (Anti-IL-1 beta antibody [EPR16805-15] ab234437).
Blocking and dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-IL-1 beta antibody [EPR16805-15] (Anti-IL-1 beta antibody [EPR16805-15] ab234437) at 1/1000 dilution
Lanes 1 and 3: Untreated NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg
Lane 2: NR8383 (rat lung macrophage (alveolar)) treated with 100ng/ml IFN gamma and 10ng TNF alpha for 32 hours, then with 5 µg/ml Brefeldin A (BFA) added for 6 hours whole cell lysate at 20 µg
Lane 4: NR8383 (rat lung macrophage (alveolar)) treated with 100ng/ml LPS and 300ng /ml Brefeldin A (BFA) for 6 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 20 kDa, 31 kDa
Exposure time: 40s
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