anti-IL-1 beta antibody [EPR18815-27] ab242561 is a carrier free rabbit monoclonal antibody that is used in IL-1 beta sELISA. Suitable for human and monkey samples.
- Create your own immunoassay with sensitive, specific matched antibody pairs
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
Images
![Sandwich ELISA - Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) (AB242561), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr18815-27-bsa-and-azide-free-capture-ab242561--sandwich-elisa-img400264.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) (AB242561)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
| Monkey | Expected |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).
Alternative names
IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin
Recommended products
anti-IL-1 beta antibody [EPR18815-27] ab242561 is a carrier free rabbit monoclonal antibody that is used in IL-1 beta sELISA. Suitable for human and monkey samples.
- Create your own immunoassay with sensitive, specific matched antibody pairs
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18815-27
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab242561 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Monkey IL-1 beta. The recommended pair for sandwich ELISA is:
Capture: ab242561, Human/Monkey IL-1 beta Capture Antibody (unconjugated)
Detector: Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector) ab242986, Human/Monkey IL-1 beta Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml for human and 28.1 - 1800 pg/ml for monkey.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) (ab242561), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-epr18815-27-bsa-and-azide-free-capture-ab242561--sandwich-elisa-img400264.jpg "Sandwich ELISA - Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) (ab242561)")
Sandwich ELISA - Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) (ab242561)
Background subtracted standard curve using Recombinant Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) (ab242561), Recombinant Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector) (Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector) ab242986), and Recombinant human IL-1 beta protein (Active) (Recombinant human IL-1 beta protein (Active) ab259387) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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