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Rabbit Recombinant Monoclonal IL-1 beta antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 34 publications.


Images

Western blot - Anti-IL-1 beta antibody [EPR21086] (AB216995), expandable thumbnail
  • Western blot - Anti-IL-1 beta antibody [EPR21086] (AB216995), expandable thumbnail
  • Immunoprecipitation - Anti-IL-1 beta antibody [EPR21086] (AB216995), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR21086] (AB216995), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info
1/30
Notes

-

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Tested
Tested

Species

Human

Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal IL-1 beta antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 34 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21086

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-IL-1 beta antibody [EPR21086] (ab216995), expandable thumbnail

    Western blot - Anti-IL-1 beta antibody [EPR21086] (ab216995)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    The expression profile is consistent with the literature (PMID 15192144 and 10845914).

    All lanes: Western blot - Anti-IL-1 beta antibody [EPR21086] (ab216995) at 1/1000 dilution

    Lane 1: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: THP-1 treated with 100 ng/ml Lipopolysaccharide (LPS) for 3 hours at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa

    Exposure time: 3min

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    The expression profile is consistent with the literature (PMID 15192144 and 10845914).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216995).

  • Western blot - Anti-IL-1 beta antibody [EPR21086] (ab216995), expandable thumbnail

    Western blot - Anti-IL-1 beta antibody [EPR21086] (ab216995)

    Lanes 1 - 4: Merged signal (red and green). Green - ab216995 observed at 27-32 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab216995 was shown to react with IL-1 beta in LPS-treated wild-type THP-1 cells in Western blot with loss of signal observed in IL1B knockout cell line Human IL1B knockout THP-1 cell line ab273762 (LPS-treated) (knockout cell lysate Human IL1B knockout THP-1 cell lysate ab275508). Wild-type THP-1 and IL1B knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab216995 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-IL-1 beta antibody [EPR21086] (ab216995) at 1/1000 dilution

    Lane 1: Wild-type THP-1 untreated cell lysate at 30 µg

    Lane 2: Wild-type THP-1 LPS-treated (3 h, 100 ng/ml) cell lysate at 30 µg

    Lane 3: IL1B knockout THP-1 untreated cell lysate at 30 µg

    Lane 4: IL1B knockout THP-1 LPS-treated (3 h, 100 ng/ml) cell lysate at 30 µg

    Performed under reducing conditions.

    Predicted band size: 30 kDa

    Observed band size: 27-32 kDa

  • Immunoprecipitation - Anti-IL-1 beta antibody [EPR21086] (ab216995), expandable thumbnail

    Immunoprecipitation - Anti-IL-1 beta antibody [EPR21086] (ab216995)

    IL-1 beta was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours, with ab216995 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216995 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.


    Lane 1: THP-1 (human monocytic leukemia monocyte) treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours whole cell lysate 10 μg (Input).
    Lane 2: ab216995 IP in THP-1 treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab216995 in THP-1 treated with 100 ng/ml lipopolysaccharide (LPS) for 3 hours whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-IL-1 beta antibody [EPR21086] (ab216995)

    Predicted band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR21086] (ab216995), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR21086] (ab216995)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilised U-937 (human histiocytic lymphoma monocyte) cells labeling IL-1 beta with ab216995 at 1/50 (10 μg/ml) dilution. Followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 1/200 (2.5 μg/ml) dilution. Nuclear staining: DAPI.

    Confocal image showing cytoplasmic staining in U-937 cells treated with Phorbol-12-myristate-13-acetate (100 nM) for 2 days, then replaced it with Lipopolysaccharides (1 μg/ml) for 13 h, with addition of Brefeldin A (5 μg/ml) for the last 4 hours.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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