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AB323249

Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free

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Chicken Recombinant Monoclonal IL-1 beta antibody. Carrier free. Suitable for ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

Interleukin-1 beta, IL-1 beta, Il1b

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249)

ab323240 staining IL1B in wild-type THP-1 cells and IL1B knockout THP-1 cells +/- TPA (80nM overnight) and LPS (100ng/ml, 6h) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with ab323240 at 1ug/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This data was developed using the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249)

ab323240 staining IL1B in NR8383 +/- 100ng/ml LPS 7h, with 1ug/ml Brefeldin A for last 3h cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with ab323240 at 5ug/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This data was developed using the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249)

ab323240 staining IL1B in Raw264.7 +/- 100ng/ml LPS 7h, with 1ug/ml Brefeldin A for last 3h cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with ab323240 at 5ug/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This data was developed using the same antibody clone in a different buffer formulation.

  • Unconjugated

    Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric)

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

EPR23851-127

Isotype

IgY

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

IL-1 beta is not present under homeostatic conditions and it is induced and secreted only upon inflammatory signals. Stimulation may be required for the detection of IL-1β as it is not constitutively expressed no matter in precursor or mature form. For example when using ab254360 positive signal can be detected in RAW264.7 with 100 ng/ml LPS treatment for 7h and additional 300 ng/ml Brefeldin A treatment in the last 3h. IL-1β is a secreted protein it is recommended to treat cells with anti-secretion reagents (eg. Brefeldin A) that inhibit secretion.

FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the Product Protocol section.

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Complementary Products

Assay Kits

AB46052

Human IL-1 beta ELISA Kit

ELISA - Human IL-1 beta ELISA Kit (AB46052)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab254360). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

ab323249 is the carrier-free version of ab323240

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Thiophilic Resin
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
See full target information Il1b
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com