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Chicken Recombinant Monoclonal IL-1 beta antibody. Carrier free. Suitable for ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) – BSA and Azide Free (AB323249), expandable thumbnail

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

Alternative names

Chicken Recombinant Monoclonal IL-1 beta antibody. Carrier free. Suitable for ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgY

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR23851-127

Purification technique

Affinity purification Thiophilic Resin

Specificity

IL-1 beta is not present under homeostatic conditions and it is induced and secreted only upon inflammatory signals. Stimulation may be required for the detection of IL-1βas it is not constitutively expressed no matter in precursor or mature form. For example when using ab254360 positive signal can be detected in RAW264.7 with 100 ng/ml LPS treatment for 7h and additional 300 ng/ml Brefeldin A treatment in the last 3h. IL-1β is a secreted protein it is recommended to treat cells with anti-secretion reagents (eg. Brefeldin A) that inhibit secretion.

FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the support & downloads section.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-IL-1 beta antibody [EPR23851-127] ab254360). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

ab323249 is the carrier-free version of Anti-IL-1 beta antibody [EPR23851-127] – Chicken IgY (Chimeric) ab323240

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com