Mouse Monoclonal IL-1 beta antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL1B.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (AB156791), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-oti3e1-ab156791--immunocytochemistry-immunofluorescence-img186132.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (AB156791)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (AB156791), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-oti3e1-ab156791--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (AB156791)")
Publications
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- Archives of Razi Institute 77:2299-23062022Molecular Interplay of Virus with Interleukin 1 Beta in Colorectal Carcinomatous Tissues from a Group of Iraqi Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZ Muhsen M Ali,H Ghaith Sachit,M Jaafar Hussein,S H Mohammed Ali,S H Mohammed Al-Alwany,A Khalil HusseinPubMed 37274872
- Experimental and therapeutic medicine 22:10812021Herbal melanin induces interleukin-1β secretion and production by human THP-1 monocytes via Toll-like receptor 2 and p38 MAPK activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdila El-Obeid,Wesam Bin Yahya,Bader Almuzzaini,Abeer Al Tuwaijri,Maria Najdi,Adil Hassib,Sabine Matou-NasriPubMed 34447474
- Journal of neuroscience research 99:2721-27422021Trigeminal neuropathy causes hypomyelination in the anterior cingulate cortex, disrupts the synchrony of neural circuitry, and impairs decision-making in male rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuresh K Murugappan,Mahadi Hasan,Zhuogui Lei,Zafar Iqbal,Aruna S Ramkrishnan,Heung Y Wong,Ying LiPubMed 34323312
- Journal of hepatocellular carcinoma 8:599-6112021Blocking the CCL5-CCR5 Axis Using Maraviroc Promotes M1 Polarization of Macrophages Cocultured with Irradiated Hepatoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Zhuang,Xiaomei Zhao,Baoying Yuan,Zhaochong Zeng,Yixing ChenPubMed 34178876
- ASN neuro 13:175909142110032472021LncRNA TUG1 Demethylated by TET2 Promotes NLRP3 Expression, Contributes to Cerebral Ischemia/Reperfusion Inflammatory Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Yin,Wei-Ping Chen,Xiao-Ping Yin,Jiang-Long Tu,Na Hu,Zheng-Yu LiPubMed 33853366
- Journal of materials science. Materials in medicin 31:702020The effect of macrophages on an atmospheric pressure plasma-treated titanium membrane with bone marrow stem cells in a model of guided bone regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoto Toyama et. al.PubMed 32705350
- Experimental physiology 105:966-9782020Knockout of MD1 contributes to sympathetic hyperactivity and exacerbates ventricular arrhythmias following heart failure with preserved ejection fraction via NLRP3 inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Jie Yang,Bin Kong,Wei Shuai,Jing-Jing Zhang,He HuangPubMed 32240565
- Cancer discovery 10:1210-12252020Myeloid-Derived Suppressor Cell Subsets Drive Glioblastoma Growth in a Sex-Specific Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDefne Bayik et. al.PubMed 32300059
- International journal of oncology 54:1981-19942019Overexpressing miR‑335 inhibits DU145 cell proliferation by targeting early growth response 3 in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Zhang et. al.PubMed 31081063
- Oncology letters 16:2606-26122018promotes the progression of colorectal cancer by interacting with E-cadherin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Ting Ma et. al.PubMed 30013655
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IL1B. Database link P01584
Reactivity data
Select an application| ICC/IF | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.4 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/150 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).
Alternative names
IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin
Recommended products
Mouse Monoclonal IL-1 beta antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL1B.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IL1B. Database link P01584
- Clone number
- OTI3E1
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from TCS
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The clone number has been updated from 3E1 to OTI3E1, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (ab156791), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-oti3e1-ab156791--immunocytochemistry-immunofluorescence-img186132.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (ab156791)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (ab156791)
ab156791 staining IL-1 beta in wild-type THP-1 cells and IL1B knockout THP-1 cells (Human IL1B knockout THP-1 cell line ab273762) +/- TPA (80nM overnight) and LPS (100ng/ml, 6h). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab156791 at 0.4μg/ml concentration and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (ab156791), expandable thumbnail](https://content.abcam.com/products/images/il-1-beta-antibody-oti3e1-ab156791--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133180.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (ab156791)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (ab156791)
Immunohistochemical staining of paraffin-embedded Human endometrium adenocarcinoma tissue labelleing IL-1B using ab156791 at 1/150 dilution.
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