AB156791

Anti-IL-1 beta antibody [OTI3E1]

KO Validated
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(23 Publications)

Mouse Monoclonal IL-1 beta antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL1B.

View Alternative Names

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (AB156791)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (AB156791)

ab156791 staining IL-1 beta in wild-type THP-1 cells and IL1B knockout THP-1 cells (ab273762) +/- TPA (80nM overnight) and LPS (100ng/ml, 6h). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab156791 at 0.4μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor^® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor^® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (AB156791)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (AB156791)

Immunohistochemical staining of paraffin-embedded Human endometrium adenocarcinoma tissue labelleing IL-1B using ab156791 at 1/150 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3E1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IL1B.

P01584

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.4 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" } } }

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Product details

The clone number has been updated from 3E1 to OTI3E1, both clone numbers name the same clone.

This product was changed from ascites to tissue culture supernatant on 6^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).

See full target information IL1B

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

APL bioengineering 9:026112 PubMed40290726

2025

Programmed wound healing in aged skin may be enhanced by mesenchymal cell loaded gene-activated scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Das,Martin Maresch,Nigamananda Dey,Noof Sulaiman,Amr Gamal Ashour,Hamad M Ammar,Mohammed Basem,Mohammed A Al Muharraqi,Matthew McGrath,Melvin Varghese Jacob,Fergal J O'Brien,Michael B Keogh

Endocrine connections 14: PubMed39621557

2024

Iodine activates NLRP3 inflammasomes in PBMCs of patients with autoimmune thyroiditis and regulates Th1 and Th17 cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wu,Qingling Guo,Yongping Liu,Xun Gong,Wei Sun,Yushu Li,Chenling Fan,Weiping Teng,Zhongyan Shan

EBioMedicine 108:105342 PubMed39321499

2024

Cellular and molecular determinants of bacterial burden in leprosy granulomas revealed by single-cell multimodal omics.

Applications

Unspecified application

Species

Unspecified reactive species

Zihao Mi,Zhenzhen Wang,Yi Wang,Xiaotong Xue,Xiaojie Liao,Chuan Wang,Lele Sun,Yingjie Lin,Jianwen Wang,Dianhao Guo,Tingting Liu,Jianjun Liu,Robert L Modlin,Hong Liu,Furen Zhang

Journal of translational medicine 22:659 PubMed39010173

2024

miR-223 accelerates lipid droplets clearance in microglia following spinal cord injury by upregulating ABCA1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilin Ou,Yongquan Cheng,Hao Ma,Kai Chen,Qiong Lin,Jiayu Chen,Ruqin Guo,Zhiping Huang,Qixian Cheng,Nima Alaeiilkhchi,Qingan Zhu,Zucheng Huang,Hui Jiang

Journal of inflammation research 17:137-152 PubMed38223424

2024

Immune-Related Genes and Immune Cell Infiltration Characterize the Maturation Status of Arteriovenous Fistulas: An Integrative Bioinformatics Study and Experimental Validation Based on Transcriptome Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Lu,Tun Wang,Zicheng Wan,Mo Wang,Yang Zhou,Zhenyu He,Sheng Liao,Haiyang Liu,Chang Shu

Atherosclerosis 365:15-24 PubMed36646016

2023

Vascular smooth muscle cells in low SYNTAX scores coronary artery disease exhibit proinflammatory transcripts and proteins correlated with IL1B activation.

Applications

Unspecified application

Species

Unspecified reactive species

Rajkumar Dorajoo,Mario Octavianus Ihsan,Wenting Liu,Hwee Ying Lim,Veronique Angeli,Sung-Jin Park,Joyce M S Chan,Xiao Yun Lin,Mei Shan Ong,Umamaheswari Muniasamy,Chi-Hang Lee,Rijan Gurung,Hee Hwa Ho,Roger Foo,Jianjun Liu,Theo Kofidis,Chuen Neng Lee,Vitaly A Sorokin

Archives of Razi Institute 77:2299-2306 PubMed37274872

2022

Molecular Interplay of Virus with Interleukin 1 Beta in Colorectal Carcinomatous Tissues from a Group of Iraqi Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Z Muhsen M Ali,H Ghaith Sachit,M Jaafar Hussein,S H Mohammed Ali,S H Mohammed Al-Alwany,A Khalil Hussein

Nature communications 13:7658 PubMed36496445

2022

Sirt6 attenuates chondrocyte senescence and osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Liang Ji,Hua Jiang,Zhuang Li,Rui Geng,Jun Zheng Hu,Yu Cheng Lin,Jun Lu

Journal of neuroscience research 99:2721-2742 PubMed34323312

2021

Trigeminal neuropathy causes hypomyelination in the anterior cingulate cortex, disrupts the synchrony of neural circuitry, and impairs decision-making in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh K Murugappan,Mahadi Hasan,Zhuogui Lei,Zafar Iqbal,Aruna S Ramkrishnan,Heung Y Wong,Ying Li

Experimental and therapeutic medicine 22:1081 PubMed34447474

2021

Herbal melanin induces interleukin-1β secretion and production by human THP-1 monocytes via Toll-like receptor 2 and p38 MAPK activation.

Applications

Unspecified application

Species

Unspecified reactive species

Adila El-Obeid,Wesam Bin Yahya,Bader Almuzzaini,Abeer Al Tuwaijri,Maria Najdi,Adil Hassib,Sabine Matou-Nasri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-1 beta antibody [OTI3E1]

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 beta antibody [OTI3E1] (AB156791)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [OTI3E1] (AB156791)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (23)

Product promise