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AB134742

Anti-IL-10 antibody [4A7-25-17]

4

(6 Reviews)

|

(15 Publications)

Mouse Monoclonal IL-10 antibody. Suitable for WB, sELISA, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL10.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images
Sandwich ELISA - Anti-IL-10 antibody [4A7-25-17] (AB134742)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IL-10 antibody [4A7-25-17] (AB134742)

In a sandwich ELISA (assuming 100 μL/well), a concentration of 8.0-10.0 μg/mL of ab134742 will detect at least 2000 pg/mL of Recombinant Human IL-10 when used with Biotin Anti-IL-10 antibody (ab84031) as the detection antibody at a concentration of approximately 0.05-0.10 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4A7-25-17

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, sELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IL10.

P22301

Reactivity data

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Product details

Although some customers have been successful in IHC we no longer batch test in this application.

Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute at 1 mg/mL in water
Purification technique
Affinity purification Protein A
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 15: PubMed39860026

2025

Human Defence Factors in Different Gestational Week Placenta: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Andris Kamergrauzis,Mara Pilmane,Anna Junga

Journal of oncology 2022:9514697 PubMed35813857

2022

Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization by Regulating PTEN/PI3K Signaling in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingkun Zhao,Aobo Zhuang,Yuan Fang

Immunity 55:1118-1134.e8 PubMed35447093

2022

Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Sizun Jiang,Chi Ngai Chan,Xavier Rovira-Clavé,Han Chen,Yunhao Bai,Bokai Zhu,Erin McCaffrey,Noah F Greenwald,Candace Liu,Graham L Barlow,Jason L Weirather,John Paul Oliveria,Tsuguhisa Nakayama,Ivan T Lee,Matthias S Matter,Anne E Carlisle,Darci Philips,Gustavo Vazquez,Nilanjan Mukherjee,Kathleen Busman-Sahay,Michael Nekorchuk,Margaret Terry,Skyler Younger,Marc Bosse,Janos Demeter,Scott J Rodig,Alexandar Tzankov,Yury Goltsev,David Robert McIlwain,Michael Angelo,Jacob D Estes,Garry P Nolan

Pathology 54:409-416 PubMed34872754

2021

PD-1/PD-L1, Treg-related proteins, and tumour-infiltrating lymphocytes are associated with the development of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Omar Kujan,Muhamed Agag,Monika Smaga,Yash Vaishnaw,Majdy Idrees,Kate Shearston,Camile S Farah

Journal of reproductive immunology 145:103314 PubMed33836321

2021

The effect of Gestational Diabetes Mellitus on the fetal compartment.

Applications

Unspecified application

Species

Unspecified reactive species

Thiago P B De Luccia,Erika Ono,Ramkumar Menon,Alexandre Urban Borbely,Rosiane Mattar,Lauren Richardson,Ana Lucia Mendes da Silva,Rayane Martins Botelho,Maria Luisa Toledo Leite Ferreira da Rocha,Silvia Daher

Medicine 100:e24619 PubMed33578573

2021

Prediction and identification of CD4+ T cell epitope for the protective antigens of Mycobacterium tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Xuefeng Chen,Ju Wang,Fang Wu,Jie Zhang,Jiangtao Dong,Hui Zhang,Xiaoling Liu,Na Hu,Jiangdong Wu,Le Zhang,Wei Cheng,Chunjun Zhang,Wan Jiang Zhang

American journal of reproductive immunology (New York, N.Y. : 1989) 82:e13181 PubMed31420980

2019

Intrauterine inhibition of chemokine receptor 4 signaling modulates local and systemic inflammation in ovine pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Stacia Z McIntosh,Clara J Maxam,Marlie M Maestas,Kelsey E Quinn,Ryan L Ashley

Chinese medical journal 132:1524-1532 PubMed31205078

2019

Effect of the interleukin 10 polymorphisms on interleukin 10 production and visceral hypersensitivity in Chinese patients with diarrhea-predominant irritable bowel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Wei Zhu,Zuo-Jing Liu,Qing-Hua Sun,Li-Ping Duan

Clinical and experimental dental research 5:377-388 PubMed31944625

2019

The relationship between T-helper cell polarization and the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Sheng Bi,Li-Juan Sun,Hong-Lei Qu,Fang Chen,Bei-Min Tian,Fa-Ming Chen

Oncology reports 41:501-511 PubMed30320402

2018

In vitro neutralization of autocrine IL‑10 affects Op18/stathmin signaling in non‑small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Sangyan Chen,Fang Shen,Dan Long,Ting Yu,Mengru Wu,Xuechi Lin
View all publications

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