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Mouse Monoclonal IL-10 antibody. Suitable for WB, sELISA, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL10.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Monoclonal
- Immunogen
Recombinant Full Length Protein corresponding to Human IL10. Database link: P22301
Reactivity data
Select an application| WB | sELISA | IHC-P | |
|---|---|---|---|
| Human | Expected | Predicted | Expected |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
Images
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. We recommend 3% Nonfat Dry Milk in diH2O as a blocking solution |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 8.00000-10.00000 µg/mL | Notes 8.0-10.0 μg/ml of this antibody will detect at least 1000 pg/well of recombinant Human IL-10. As the detection antibody at a concentration of approximately 0.5-1.0 μg/ml. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Customer feedback suggests heat-mediated antigen retrieval with Tris-EDTA pH9.0 is effective. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal IL-10 antibody. Suitable for WB, sELISA, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL10.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Monoclonal
- Immunogen
Recombinant Full Length Protein corresponding to Human IL10. Database link: P22301
- Clone number
4A7-25-17
- Purification technique
Affinity purification Protein A
- Light chain type
kappa
- Reconstitution
This product can be reconstituted to a final concentration of 1 mg/ml in water.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Although some customers have been successful in IHC we no longer batch test in this application.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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