AB192271

Anti-IL-10 antibody

Name: Anti-IL-10 antibody (ab192271) | Abcam
Brand: Abcam Limited
SKU: AB192271
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(15 Publications)

Rabbit Polyclonal IL-10 antibody. Suitable for WB and reacts with Rat samples. Cited in 15 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Il10.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

1 Images

Supplier Data

Western blot - Anti-IL-10 antibody (AB192271)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab192271 at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT.

All lanes:

Western blot - Anti-IL-10 antibody (ab192271) at 0.5 µg/mL

Lane 1:

Recombinant rat IL10 protein at 10 ng

Lane 2:

Recombinant rat IL10 protein at 5 ng

Lane 3:

Recombinant rat IL10 protein at 1 ng

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Il10.

P29456

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information Il10

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 11:197 PubMed37007562

2023

Inhibition of NF-κB ameliorates aberrant retinal glia activation and inflammatory responses in streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Ding,Zhongcui Sun,Yue Guo,Wenyi Tang,Qinmeng Shu,Gezhi Xu

Evidence-based complementary and alternative medicine : eCAM 2022:7971801 PubMed36317105

2022

Acupuncture Inhibits Morphine Induced-Immune Suppress via Antioxidant System.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Jie Zhao,Dae Geon Lee,Chan Sik Park,Chae Ha Yang,Hee Young Kim,Mi Young Lee,Chang-Hyun Song,Il Je Cho,Sang Chan Kim,Sae Kwang Ku,Bong Hyo Lee

Frontiers in immunology 13:873382 PubMed35720361

2022

Adiponectin Ameliorates GMH-Induced Brain Injury by Regulating Microglia M1/M2 Polarization AdipoR1/APPL1/AMPK/PPARγ Signaling Pathway in Neonatal Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ningbo Xu,Xifeng Li,Jun Weng,Chunhua Wei,Zhenyan He,Desislava Met Doycheva,Cameron Lenahan,Wenhui Tang,Jian Zhou,Yanchao Liu,Qiang Xu,Yahong Liu,Xuying He,Jiping Tang,John H Zhang,Chuanzhi Duan

Cell death & disease 12:826 PubMed34480017

2021

Expansion of monocytic myeloid-derived suppressor cells ameliorated intestinal inflammatory response by radiation through SOCS3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

You Yeon Choi,Ki Moon Seong,Hyun Jung Lee,Seung Sook Lee,Areumnuri Kim

Frontiers in medicine 8:649654 PubMed34307396

2021

STAT3 but Not STAT5 Contributes to the Protective Effect of Electroacupuncture Against Myocardial Ischemia/Reperfusion Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Hui Guo,Xin-Yue Jing,Hui Chen,Hou-Xi Xu,Bing-Mei Zhu

Aging 12:26029-26046 PubMed33361528

2020

DUSP5 suppresses interleukin-1β-induced chondrocyte inflammation and ameliorates osteoarthritis in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Wu,Langhai Xu,Yuzhe He,Kai Xu,Zhonggai Chen,Safwat Adel Abdo Moqbel,Chiyuan Ma,Lifeng Jiang,Jisheng Ran,Lidong Wu,Ying Zhong

Scientific reports 10:20185 PubMed33214576

2020

Different experimental multiple trauma models induce comparable inflammation and organ injury.

Applications

Unspecified application

Species

Unspecified reactive species

Borna Relja,Bing Yang,Katrin Bundkirchen,Baolin Xu,Kernt Köhler,Claudia Neunaber

PLoS pathogens 16:e1008474 PubMed32315358

2020

PARP1-cGAS-NF-κB pathway of proinflammatory macrophage activation by extracellular vesicles released during Trypanosoma cruzi infection and Chagas disease.

Applications

Unspecified application

Species

Unspecified reactive species

Subhadip Choudhuri,Nisha Jain Garg

Theranostics 10:5412-5434 PubMed32373221

2020

Identification of a novel microRNA-141-3p/Forkhead box C1/β-catenin axis associated with rheumatoid arthritis synovial fibroblast function in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Yin Wang,Hui Zhang,Jun Chang,Ming Lu,Weilu Gao,Wendong Liu,Yetian Li,Li Yin,Xiaohe Wang,Yuejun Wang,Mengru Gao,Zongsheng Yin

Brain research bulletin 150:127-135 PubMed31129170

2019

Interleukin-33 reduces neuronal damage and white matter injury via selective microglia M2 polarization after intracerebral hemorrhage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouqing Chen,Na Xu,Xuejiao Dai,Chongshun Zhao,Xin Wu,Sandhya Shankar,Huachen Huang,Zhong Wang

View all publications

style

left-column