Rabbit Polyclonal IL-10 antibody. Suitable for ELISA, WB, RIA, Neut and reacts with Recombinant fragment, Human, Recombinant fragment - Human samples. Cited in 91 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human IL10.
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Publications
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- Viruses 15:2023EBV Impact in Peripheral Macrophages' Polarization Cytokines in Pediatric Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgustina Moyano,Natalia Ferressini Gerpe,Maria Eugenia Amarillo,Elena De Matteo,Maria Victoria Preciado,Maria Soledad Caldirola,Paola ChabayPubMed 37896882
- Pathogens (Basel, Switzerland) 12:2023Curcumin and Its Supramolecular Complex with Disodium Glycyrrhizinate as Potential Drugs for the Liver Fluke Infection Caused by .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria N Lvova,Denis V Ponomarev,Alena A Tarasenko,Anna V Kovner,Galina A Minkova,Michail A Tsyganov,Meijun Li,Yan Lou,Veronica I Evseenko,Alexander V Dushkin,Irina V Sorokina,Tatiana G Tolstikova,Viatcheslav A Mordvinov,Damira F AvgustinovichPubMed 37375509
- Research (Washington, D.C.) 6:00562023Restoration of Motor Function through Delayed Intraspinal Delivery of Human IL-10-Encoding Nucleoside-Modified mRNA after Spinal Cord Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLászló Gál,Tamás Bellák,Annamária Marton,Zoltán Fekécs,Drew Weissman,Dénes Török,Rachana Biju,Csaba Vizler,Rebeka Kristóf,Mitchell B Beattie,Paulo J C Lin,Norbert Pardi,Antal Nógrádi,Krisztián PajerPubMed 36930811
- Frontiers in immunology 14:10862322023Chitosan oligosaccharide improves ovarian granulosa cells inflammation and oxidative stress in patients with polycystic ovary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Xie,Wenli Hong,Yuan Li,Shuyi Ling,Ziqiong Zhou,Yuqing Dai,Wenbo Wu,Ruoxin Weng,Zhisheng Zhong,Jun Tan,Yuehui ZhengPubMed 36936973
- Parasite immunology 45:e129712023Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen M Sandoval Pacheco,Gabriela V Araujo Flores,Aurea F Ferreira,Vânia L R da Matta,Claudia M de Castro Gomes,Wilfredo H Sosa-Ochoa,Concepción Zúniga,Fernando T Silveira,Carlos E P Corbett,Márcia D LaurentiPubMed 36695719
- TheScientificWorldJournal 2022:67408532022The Cytokine and Bone Protein Expression by Ellagic Acid-Hydroxyapatite in Bone Remodelling Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDyah Noviana Primasari,Intan Nirwana,Hendrik Setia Budi,Agung Satria Wardhana,Annisa Fitria Sari,Novita Novita,Andika Putri Setyawan,Meircurius Dwi Condro Surboyo,Khairul Anuar ShariffPubMed 36561943
- Frontiers in immunology 13:9409102022PD-L1 is upregulated in CD163+ tonsillar macrophages from children undergoing EBV primary infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgustina Moyano,Natalia Ferressini,Elena De Matteo,Maria Victoria Preciado,Paola ChabayPubMed 36451810
- Frontiers in immunology 13:9484572022Fine-tuning of microglia polarization prevents diabetes-associated cerebral atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Zhu,Pengfei Xing,Ping Zhang,Minmin Zhang,Hongjian Shen,Lei Chen,Fang Shen,Yi Jiang,Hui Yuan,Lei Zhang,Jing Wang,Xiongfeng Wu,Yu Zhou,Tao Wu,Benqiang Deng,Jianmin Liu,Yongwei Zhang,Pengfei YangPubMed 35935990
- Frontiers in bioengineering and biotechnology 10:9028942022Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Ying Teng,Ming-Li Zou,Si-Yu Liu,Yuan Jia,Kai-Wen Zhang,Zheng-Dong Yuan,Jun-Jie Wu,Jun-Xing Ye,Shun Yu,Xia Li,Xiao-Jin Zhou,Feng-Lai YuanPubMed 35832407
- Journal of applied microbiology 133:908-9212022Microbial and metabolic profiles of bronchopulmonary dysplasia and therapeutic effects of potential probiotics Limosilactobacillus reuteri and Bifidobacterium bifidum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Li,Li He,Qin Zhao,Tao BoPubMed 35488863
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human IL10. Database link P22301
Consider this alternative
Reactivity data
Select an application| ELISA | WB | RIA | Neut | |
|---|---|---|---|---|
| Human | Expected | Predicted | Expected | Expected |
| Recombinant fragment | Expected | Not recommended | Expected | Expected |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes (as detection antibody, using a monoclonal antibody for capture). |
Species Human | Dilution info - | Notes (as detection antibody, using a monoclonal antibody for capture). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/2000 - 1/10000 | Notes In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
RIA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
Alternative names
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Recommended products
Rabbit Polyclonal IL-10 antibody. Suitable for ELISA, WB, RIA, Neut and reacts with Recombinant fragment, Human, Recombinant fragment - Human samples. Cited in 91 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human IL10.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human IL10. Database link P22301
- Purity
- Whole antiserum
- Specificity
The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-IL-10 antibody (ab34843)
4-20% reducing gel.Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.Dilution buffer: 3% BSA-TBS.Incubated O/N at 4°C.
All lanes: Western blot - Anti-IL-10 antibody (ab34843) at 1/200 dilution
All lanes: recombinant IL-10 protein
SecondaryAll lanes: Goat anti-rabbit at 1/20000 dilution
Predicted band size: 20 kDa
Western blot - Anti-IL-10 antibody (ab34843)
All lanes: Western blot - Anti-IL-10 antibody (ab34843) at 1/500 dilution
All lanes: recombinant IL-10 protein, 50ng
SecondaryAll lanes: Peroxidase conjugated secondary at 1/40000 dilution
Predicted band size: 20 kDa
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