AB34843

Anti-IL-10 antibody

Name: Anti-IL-10 antibody. Rabbit polyclonal (ab34843) | Abcam
Brand: Abcam Limited
SKU: AB34843
Rating: 4 (15 reviews)

(15 Reviews)

(107 Publications)

Rabbit Polyclonal IL-10 antibody. Suitable for ELISA, WB, RIA, FuncS (Neut/Block) and reacts with Recombinant fragment, Human, Recombinant fragment - Human samples. Cited in 107 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human IL10.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

2 Images

Supplier Data

Western blot - Anti-IL-10 antibody (AB34843)

Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.

Dilution buffer : 3% BSA-TBS.

Incubated O/N at 4°C.

All lanes:

Western blot - Anti-IL-10 antibody (ab34843) at 1/200 dilution

All lanes:

recombinant IL-10 protein

Secondary

All lanes:

Goat anti-rabbit at 1/20000 dilution

Predicted band size: 20 kDa

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Supplier Data

Western blot - Anti-IL-10 antibody (AB34843)

All lanes:

Western blot - Anti-IL-10 antibody (ab34843) at 1/500 dilution

All lanes:

recombinant IL-10 protein, 50ng

Secondary

All lanes:

Peroxidase conjugated secondary at 1/40000 dilution

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, FuncS (Neut/Block), RIA, ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human IL10.

P22301

Specificity

The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "(as detection antibody, using a monoclonal antibody for capture).", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "" }, "Recombinant fragment": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "(as detection antibody, using a monoclonal antibody for capture).", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "" }, "Recombinant fragment - Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "FuncSNeutBlock-species-checked": "notRecommended", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.01% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information IL10

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Publications (107)

Recent publications for all applications. Explore the full list and refine your search

Parasite immunology 47:e70026 PubMed40947759

2025

In Situ Evaluation of Macrophage Populations and Inflammasome Components in Cutaneous and Mucocutaneous Leishmaniasis.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline de Heleno Chagas de Carvalho,Gabriela Venicia Araujo Flores,Carmen Maria Sandoval Pacheco,Vania Lucia da Ribeiro da Matta,Carolina de Esteves de Morais,Ricardo Romiti,Walter Belda Júnior,Márcia Dalastra Laurenti

International journal of molecular sciences 25: PubMed39518925

2024

Evaluation of the Effect of an α-Adrenergic Blocker, a PPAR-γ Receptor Agonist, and a Glycemic Regulator on Chronic Kidney Disease in Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Morones,Mariana Pérez,Martín Muñoz,Esperanza Sánchez,Manuel Ávila,Jorge Topete,Javier Ventura,Sandra Martínez

European journal of medical research 29:450 PubMed39223620

2024

Ginsenoside Rh2 suppresses ferroptosis in ulcerative colitis by targeting specific protein 1 by upregulating microRNA-125a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Zhao,WenQiang Yuan,LiuChan Yang,Fang Yan,DeJun Cui

Frontiers in immunology 15:1386528 PubMed38590527

2024

The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Zdenek Tauber,Adela Burianova,Katerina Koubova,Max Mrstik,Marie Jirkovska,Katerina Cizkova

BMC complementary medicine and therapies 24:101 PubMed38402163

2024

JianPiYiShen formula prevents cisplatin-induced acute kidney injury in mice by improving necroptosis through MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongtang Li,Riming He,Jiahui Liu,Xiaoming Jin,Beibei Jiang,Yunlan Lao,Shudong Yang

Scientific reports 14:4497 PubMed38402335

2024

High-voltage pulsed radiofrequency improves ultrastructure of DRG and enhances spinal microglial autophagy to ameliorate neuropathic pain induced by SNI.

Applications

Unspecified application

Species

Unspecified reactive species

Ri Chen,Xueru Xu,Youfen Yu,Yanqin Chen,Chun Lin,Rongguo Liu

Scientific reports 14:2135 PubMed38273012

2024

Tonsillar cytotoxic CD4 T cells are involved in the control of EBV primary infection in children.

Applications

Unspecified application

Species

Unspecified reactive species

María Eugenia Amarillo,Agustina Moyano,Natalia Ferressini Gerpe,Elena De Matteo,Maria Victoria Preciado,Paola Chabay

Heliyon 10:e23635 PubMed38187273

2024

High species homology potentiates quantitative inflammation profiling in zebrafish using immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

T Ollewagen,R M Benecke,C Smith

Viruses 15: PubMed37896882

2023

EBV Impact in Peripheral Macrophages' Polarization Cytokines in Pediatric Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Agustina Moyano,Natalia Ferressini Gerpe,Maria Eugenia Amarillo,Elena De Matteo,Maria Victoria Preciado,Maria Soledad Caldirola,Paola Chabay

Pathogens (Basel, Switzerland) 12: PubMed37375509

2023

Curcumin and Its Supramolecular Complex with Disodium Glycyrrhizinate as Potential Drugs for the Liver Fluke Infection Caused by .

Applications

Unspecified application

Species

Unspecified reactive species

Maria N Lvova,Denis V Ponomarev,Alena A Tarasenko,Anna V Kovner,Galina A Minkova,Michail A Tsyganov,Meijun Li,Yan Lou,Veronica I Evseenko,Alexander V Dushkin,Irina V Sorokina,Tatiana G Tolstikova,Viatcheslav A Mordvinov,Damira F Avgustinovich

View all publications

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