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AB9969

Anti-IL-10 antibody

4

(4 Reviews)

|

(117 Publications)

Anti-IL-10 antibody (ab9969) is a rabbit polyclonal antibody detecting IL-10 in Western Blot, ICC/IF, ELISA. Suitable for Mouse, Rat.

- Over 80 publications
- Trusted since 2002

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

1 Images
Sandwich ELISA - Anti-IL-10 antibody (AB9969)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IL-10 antibody (AB9969)

Sandwich ELISA ab9969. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

No

Reacts with

Rat, Mouse

Applications

sELISA, ICC/IF, WB, ELISA

applications

Immunogen

Recombinant Fragment Protein within Rat Il10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29456

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Reactivity data

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Product details

What is this antibody validated in?
Anti-IL-10 antibody (ab9969) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Mouse, Rat samples.

Trusted by the scientific community
Anti-IL-10 (ab9969) was first used in a scientific publication in 2002 and has been cited over 80 times in peer-reviewed journals.
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Purification technique
Affinity purification Immunogen
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Storage after reconstitution: Up to two weeks at 4&deg;C or six months at -20&deg;C.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).
See full target information Il10
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Publications (117)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29297 PubMed40789880

2025

Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Sun,Yuan Gao,Kadirya Asan,Jing Ji,Jinjin Xu,Xiaoyan Wu,Jie Ren,Wen Feng,Mengwei Song,Sen Wang,Boyu Zhang,Ying Zhou,Conghui Han,Shun Liu,Wen Yang

European journal of medical research 30:676 PubMed40713698

2025

Ulinastatin inhibits macrophage M1 polarization to improve acute pancreatitis-associated intestinal barrier dysfunction by promoting Nrf2 signaling pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Jiahui Fang,Siqi Zhang,Ming Gao

Nature communications 16:6151 PubMed40610440

2025

Adipocyte CLDN5 promotes thermogenesis and energy expenditure through regulation of IL10 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Feng,Wenqin Wang,Xianlong Gao,Hejie Yan,Mengyuan Xu,Baozhen Fan,Qianfeng Jia,Chao Wang,Jian Yu,Yi Li,Qinfeng Xu,Yanan An,Peng Jiao,Mingxia Wang,Hui Sun,Feng Kong,Yongfeng Gong,Shengtian Zhao

Cellular and molecular life sciences : CMLS 82:165 PubMed40249512

2025

Involvement of miRNA-204 carried by the exosomes of macrophages in the AT2 receptor-mediated improvement of vascular calcification.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yu Bai,Xiao-Rui Lv,Hai-Bo Gu,Hui Li,Bao-Shuai Shan

Biology of sex differences 15:100 PubMed39633480

2024

Estrogen administration enhances the adverse effects of cigarette smoking on the heart in cycling female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Emna Abidi,Reine Diab,Rana Zahreddine,Ghadir Amin,Abdullah Kaplan,George W Booz,Fouad A Zouein

Scientific reports 14:29408 PubMed39592660

2024

Administration of monophosphoryl lipid A shortly after traumatic brain injury blocks the following spatial and avoidance memory loss and neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Hooshmand,Mohammad Reza Sadeghi,Ahmad Asoodeh,Hamid Gholami Pourbadie,Mahbobeh Kamrani Mehni,Mohamad Sayyah

Frontiers in pharmacology 15:1469602 PubMed39391701

2024

Neuroprotective effects of Blume on promoting M2 microglial polarization by inhibiting JNK/TLR4/T3JAM/NF-κB signaling after transient ischemic stroke in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shang-Chih Huang,Hui-Chi Huang,Wen-Ling Liao,Shung-Te Kao,Chin-Yi Cheng

The American journal of pathology 194:2194-2211 PubMed39159867

2024

Immunoregulatory Properties of Immune Cells that Associate with the Lens Capsule Surface during Acute and Resolution Phases of Experimental Autoimmune Uveitis.

Applications

Unspecified application

Species

Unspecified reactive species

Phuong M Le,Mary J Mattapallil,Rachel R Caspi,Mary Ann Stepp,A Sue Menko

Reproductive biology and endocrinology : RB&E 22:75 PubMed38926848

2024

9-cis-retinoic acid signaling in Sertoli cells regulates their immunomodulatory function to control lymphocyte physiology and Treg differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Alicja Kamińska,Laura Pardyak,Sylwia Lustofin,Karolina Gielata,Zbigniew Arent,Agnieszka Pietsch-Fulbiszewska,Anna Hejmej

Bioactive materials 39:392-405 PubMed38855060

2024

C176-loaded and phosphatidylserine-modified nanoparticles treat retinal neovascularization by promoting M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

An Shao,Lulu Jin,Yanni Ge,Ziqiang Ye,Mingyu Xu,Yifan Zhou,Yingyu Li,Linyan Wang,Pinglong Xu,Kai Jin,Zhengwei Mao,Juan Ye
View all publications
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Product promise

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