Rabbit Polyclonal IL-10 antibody. Suitable for ELISA, WB, sELISA, ICC/IF and reacts with Recombinant full length protein - Rat, Rat, Mouse samples. Cited in 88 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Il10 aa 1 to C-terminus.
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- Frontiers in pharmacology 14:11888932023The neuroprotective effects of Liuwei Dihuang medicine in the APP/PS1 mouse model are dependent on the PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Yuan,Yamei Liu,Li Hao,Jinlian Ma,Simai Shao,Ziyang Yu,Ming Shi,Zhenqiang Zhang,Zijuan ZhangPubMed 37920210
- Advanced healthcare materials 12:e23008872023Nanosized Silk-Magnesium Complexes for Tissue Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaozhao Ding,Weinan Cheng,Lutong Liu,Gang Xu,Qiang Lu,David L KaplanPubMed 37317936
- Frontiers in immunology 14:11075592023Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke the brain-gut axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiongqiong Zhang,Peiying Deng,Suhui Chen,Hong Xu,Yamin Zhang,Hui Chen,Jianmin Zhang,Hua SunPubMed 36742316
- Advanced healthcare materials 12:e22023772023GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinjin Ma,Jiaying Li,Xingran Wang,Meimei Li,Wenwen Teng,Zihan Tao,Jile Xie,Yanxia Ma,Qin Shi,Bin Li, SaijilafuPubMed 36549669
- Pharmaceutical biology 60:2189-21992022Mechanism of Huangqi-Honghua combination regulating the gut microbiota to affect bile acid metabolism towards preventing cerebral ischaemia-reperfusion injury in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Wang,Yue Chen,Jinyi Cao,Ruimin Liang,Yi Qiao,Likun Ding,Xiaojuan Yang,Zhifu YangPubMed 36307999
- Frontiers in immunology 13:10124422022IRG1/itaconate increases IL-10 release to alleviate mechanical and thermal hypersensitivity in mice after nerve injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyu Sun,Tingting Hu,Yurui Zhang,Xiaotong Wang,Jing Liu,Wen Chen,Chao Wei,Dianxin Liu,Weihua Wu,Ting Lan,Yumeng Ding,Zhaoli Luo,Meng Liu,Danmin Shen,Zhongnan Xiao,Liye Hu,Miaoyi Pang,Yiran Ma,Lei Shi,Peipei Wang,Jiannan Zhang,Qian Li,Fei YangPubMed 36311727
- Frontiers in molecular neuroscience 15:9267912022TNAP-a potential cytokine in the cerebral inflammation in spastic cerebral palsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Kun Wang,Chao Gao,He-Quan Zhong,Xiang-Yu Kong,Rui Qiao,Hui-Chun Zhang,Bai-Yun Chen,Yang Gao,Bing LiPubMed 36187348
- International journal of oncology 61:2022miR‑382 inhibits breast cancer progression and metastasis by affecting the M2 polarization of tumor‑associated macrophages by targeting PGC‑1α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Zhou,Mingyu Gan,Xin Jin,Meng Dai,Yuanyuan Wang,Youyang Lei,Zijing Lin,Jia MingPubMed 36069230
- Annals of medicine and surgery (2012) 82:1045292022Locally injected Mesenchymal Stem Cells optimize angiogenesis by regulating VEGF and CD31 expression in duodenal perforation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEko Setiawan,Bambang Purwanto,Brian Wasita,Agung PutraPubMed 36268307
- Drug delivery 29:1994-20012022Delivery of rivaroxaban and chitosan rapamycin microparticle with dual antithrombosis and antiproliferation functions inhibits venous neointimal hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Sun,Haoliang Wu,Hao He,Liwei Zhang,Yuanfeng Liu,Cong Zhang,Chunyang Lou,Jingan Li,Hualong BaiPubMed 35762638
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Il10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29456
Reactivity data
Select an application| ELISA | WB | sELISA | ICC/IF | |
|---|---|---|---|---|
| Mouse | Predicted | Expected | Predicted | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Recombinant full length protein - Rat | Tested | Not recommended | Not recommended | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584).
Alternative names
Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF
Recommended products
Rabbit Polyclonal IL-10 antibody. Suitable for ELISA, WB, sELISA, ICC/IF and reacts with Recombinant full length protein - Rat, Rat, Mouse samples. Cited in 88 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Il10 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Il10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29456
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Anti-IL-10 antibody (ab9969)
Sandwich ELISA ab9969. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.
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- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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