AB47222

Anti-IL-10 antibody [B-S10] - BSA and Azide free

Name: Anti-IL-10 antibody [B-S10] - BSA and Azide free (ab47222) | Abcam
Brand: Abcam Limited
SKU: AB47222
Price: 490 USD
Availability: InStock
Rating: 4 (1 reviews)

Advanced Validation
What is this?

(1 Review)

(1 Publication)

Mouse Monoclonal IL-10 antibody. Carrier free. Suitable for FuncS and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL10.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images

Functional Studies - Anti-IL-10 antibody [B-S10] - BSA and Azide free (AB47222)

FuncS

Unknown

Functional Studies - Anti-IL-10 antibody [B-S10] - BSA and Azide free (AB47222)

The isotype control antibody has no effect on IL-10 inhibition of IL-6 synthesis whilst the B-S10 antibody (anti-human IL-10) increases IL-6 secretion by PBMC activated LPS. B-S10 blocks the inhibition of IL-6 synthesis by IL-10 and increases IL-6 synthesis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-S10

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

FuncS

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IL10.

P22301

Specificity

This antibody recognizes both natural and recombinant IL-10.

Blocks IL-10 induced inhibition of IL-6 synthesis on LPS activated monocytes.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FuncS-species-checked": "testedAndGuaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free.

Storage buffer

pH: 7.3 - 7.5 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C|-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information IL10

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunobiology 224:497-501 PubMed31133346

2019

Synergistic effect of Dermatophagoides pteronyssinus allergens and dexamethasone on the expression of CD163 by peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paweł Leszek Bernatowicz,Agnieszka Pampuch,Paweł Golec,Ewa Sacharzewska,Michał Szczesiul,Paweł Bielecki,Krzysztof Kowal

View all publications

style

left-column

Assay Kits

AB185986

Human IL-10 ELISA Kit (Interleukin-10)

KO Validated|Recombinant|SimpleStep

ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

Primary Antibodies

AB233706

Anti-IL-6 antibody [EPR21711]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-IL-6 antibody [EPR21711] (AB233706)

Primary Antibodies

AB15323

Anti-iNOS antibody

BOND RX™ Validated

Western blot - Anti-iNOS antibody (AB15323)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com