AB52909

Anti-IL-10 antibody [EP1115Y]

RabMAb
Recombinant
20ul selling size
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal IL-10 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images

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Western blot - Anti-IL-10 antibody [EP1115Y] (AB52909)

All lanes:

Western blot - Anti-IL-10 antibody [EP1115Y] (ab52909) at 1/5000 dilution

All lanes:

Recombinant protein (5ng)

Secondary

All lanes:

Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size: 20 kDa

Observed band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1115Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "This has only been tested against the recombinant protein.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information IL10

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:12498-12509 PubMed38747738

2024

SHP2 mediates STAT3/STAT6 signaling pathway in TAM to inhibit proliferation and metastasis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guojing Chai,Yingbo Nan,Haili Zhao,Qingchuan Hu

Frontiers in cellular and infection microbiology 12:798978 PubMed35463647

2022

Junin Virus Activates p38 MAPK and HSP27 Upon Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Collin J Fitzpatrick,Rajini R Mudhasani,Louis A Altamura,Catherine E Campbell,Julie P Tran,Brett F Beitzel,Aarthi Narayanan,Cynthia L de la Fuente,Kylene Kehn-Hall,Jeffrey M Smith,Connie S Schmaljohn,Aura R Garrison

Cureus 13:e13499 PubMed33777584

2021

Proteomic Analysis of Cardioembolic and Large Artery Atherosclerotic Clots Using Reverse Phase Protein Array Technology Reveals Key Cellular Interactions Within Clot Microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Abbasi,Sean Fitzgerald,Jennifer Ayers-Ringler,Virginia Espina,Claudius Mueller,Sally Rucker,Ramanathan Kadirvel,David Kallmes,Waleed Brinjikji

PloS one 13:e0191983 PubMed29408900

2018

Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia de la Fuente,Chelsea Pinkham,Deemah Dabbagh,Brett Beitzel,Aura Garrison,Gustavo Palacios,Kimberley Alex Hodge,Emanuel F Petricoin,Connie Schmaljohn,Catherine E Campbell,Aarthi Narayanan,Kylene Kehn-Hall

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-10 antibody [EP1115Y]

Western blot - Anti-IL-10 antibody [EP1115Y] (AB52909)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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