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Rabbit Recombinant Monoclonal IL-10 antibody. Suitable for WB, Flow Cyt (Intra), IP and reacts with Mouse samples.

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Images

Western blot - Anti-IL-10 antibody [EPR27280-88] (AB310329), expandable thumbnail
  • Western blot - Anti-IL-10 antibody [EPR27280-88] (AB310329), expandable thumbnail
  • Immunoprecipitation - Anti-IL-10 antibody [EPR27280-88] (AB310329), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR27280-88] (AB310329), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IPIHC-PICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584).

Alternative names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

Recommended products

Rabbit Recombinant Monoclonal IL-10 antibody. Suitable for WB, Flow Cyt (Intra), IP and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27280-88
Purification technique
Affinity purification Protein A
Specificity

Interleukin-10 (IL-10) is an anti-inflammatory cytokine, produced mainly by monocytes/macrophages and regulatory T-cells. The expression of IL-10 is minimal in unstimulated tissues (PMID: 22438968).
When performing Western Blot (WB), ensure to add appropriate treatments such as LPS and BFA to induce and detect IL-10 expression in the RAW 264.7 cell line.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-IL-10 antibody [EPR27280-88] (ab310329), expandable thumbnail

    Western blot - Anti-IL-10 antibody [EPR27280-88] (ab310329)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    The expression of IL-10 is upregulated in response to LPS in treated RAW 264.7 (PMID: 23012430, 15218058) but not in non-differentiated NIH/3T3 cells. In western blot, anti-IL-6 antibody (Anti-IL-6 antibody [EPR23819-103] ab290735) staining at 1/1000 dilution. Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-IL-10 antibody [EPR27280-88] (ab310329) at 1/1000 dilution

    Lane 1: Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: RAW264.7 treated with 100 ng/mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate at 20 µg

    Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 treated with 100 ng/mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa

    Observed band size: 18 kDa

    Exposure time: 3min

  • Western blot - Anti-IL-10 antibody [EPR27280-88] (ab310329), expandable thumbnail

    Western blot - Anti-IL-10 antibody [EPR27280-88] (ab310329)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    The weaker band observed at 15kDa is the deglycosylated form of IL-10. In western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-IL-10 antibody [EPR27280-88] (ab310329) at 1/1000 dilution

    Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with /mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate at 20 µg

    Lane 2: RAW264.7 treated with /mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate treated with N-glycosidase F at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 18 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-IL-10 antibody [EPR27280-88] (ab310329), expandable thumbnail

    Immunoprecipitation - Anti-IL-10 antibody [EPR27280-88] (ab310329)

    IL-10 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate with ab310329 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab310329 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate
    Lane 2: ab310329 IP in RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab310329 in RAW264.7 treated with 100 ng/mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-IL-10 antibody [EPR27280-88] (ab310329) at 1/30 dilution

    All lanes: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100 ng/mL LPS for 6 hours, 300ng/mL Brefeldin A was then added for additional 3 hours whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3min

  • Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR27280-88] (ab310329), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR27280-88] (ab310329)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 6h and then add 300ng/ml BFA for 3h (middle) / untreated RAW 264.7 (Right) cells labelling IL-10 with ab310329 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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