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Rabbit Recombinant Monoclonal IL-10 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (AB324012), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (AB324012), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (AB324012), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (AB324012), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (AB324012), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Transfected cell line - Human
Tested

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

Alternative names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

Rabbit Recombinant Monoclonal IL-10 antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28980-513
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012)

    IL-10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil using rabbit Anti-IL-10 antibody

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IL-10 with ab324012 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human tonsil.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012)

    IL-10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon carcinoma using rabbit Anti-IL-10 antibody

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling IL-10 with ab324012 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon carcinoma.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012)

    IL-10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cervical carcinoma using rabbit Anti-IL-10 antibody

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling IL-10 with ab324012 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cervical carcinoma.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012)

    IL-10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human skeletal muscle using rabbit Anti-IL-10 antibody

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling IL-10 with ab324012 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human skeletal muscle.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012)

    IL-10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney using rabbit Anti-IL-10 antibody

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IL-10 with ab324012 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human kindey.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012)

    IL-10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-IL-10 antibody

    Immunohistochemical analysis of paraffin-embedded (A) Untreated HuT-78 (human Sezary syndrome cutaneous T lymphocyte) cell pellet and (B) HuT-78 treated with TPA (80nM 5h) and ionomycin (30µM 5h) add BFA (300ng/ml 4h) cell pellet labeling IL-10 with ab324012 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (B) HuT-78 treated with TPA (80nM 5h) and ionomycin (30µM 5h) add BFA (300ng/ml 4h) cell pellet, nearly no staining on (A) untreated HuT-78 cell pellet.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [EPR28980-513] (ab324012)

    IL-10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-IL-10 antibody

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with an IL10 expression vector containing a Myc-his tag cell pellet and (B) HEK-293T transfected with an empty vector containing a Myc-his tag cell pellet labeling IL-10 with ab324012 at 1/2000 (0.24 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HEK-293T transfected with an IL10 expression vector containing a Myc-his tag cell pellet. No staining on (B) HEK-293T transfected with an empty vector containing a Myc-his tag cell pellet.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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