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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rat Monoclonal IL-10 antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 42 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10.
IgG1
Rat
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
Liquid
Monoclonal
IHC-P | WB | |
---|---|---|
Mouse | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584).
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il10, Il-10
Rat Monoclonal IL-10 antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 42 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10.
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il10, Il-10
IgG1
Rat
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
Liquid
Monoclonal
Recombinant Fragment Protein within Mouse Il10. The exact immunogen used to generate this antibody is proprietary information.Database link P18893
JES5-2A5
Affinity purification Protein G
ab189392 recognizes mouse IL-10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL-10 using ab189392 at 10 μg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL-10 using ab189392 at 10 μg/ml.
All lanes: Western blot - Anti-IL-10 antibody [JES5-2A5] (AB189392) at 1/1000 dilution
All lanes: recombinant mouse IL-10
All lanes: F(ab)2 rabbit anti-rat IgG:HRP
Developed using the ECL technique.
Predicted band size: 20 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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