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Rat Monoclonal IL-10 antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 42 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (AB189392), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (AB189392), expandable thumbnail
  • Western blot - Anti-IL-10 antibody [JES5-2A5] (AB189392), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rat
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Mouse Il10. The exact immunogen used to generate this antibody is proprietary information. Database link P18893

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Associated Products

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Target data

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584).

Alternative names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

Recommended products

Rat Monoclonal IL-10 antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 42 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Mouse Il10. The exact immunogen used to generate this antibody is proprietary information. Database link P18893
Clone number
JES5-2A5
Purification technique
Affinity purification Protein G
Specificity

ab189392 recognizes mouse IL-10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL-10 using ab189392 at 10 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL-10 using ab189392 at 10 μg/ml.

  • Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392), expandable thumbnail

    Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)

    All lanes: Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392) at 1/1000 dilution

    All lanes: recombinant mouse IL-10

    Secondary

    All lanes: F(ab)2 rabbit anti-rat IgG:HRP

    Developed using the ECL technique.

    Predicted band size: 20 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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