Rabbit Recombinant Multiclonal IL-10 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Recombinant full length protein samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-10 Antibody [RP23040032] (AB313401), expandable thumbnail](https://content.abcam.com/products/images/il-10-antibody-rp23040032-ab313401--immunocytochemistry-immunofluorescence-img417971.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-10 Antibody [RP23040032] (AB313401)")
![Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401), expandable thumbnail](https://content.abcam.com/products/images/il-10-antibody-rp23040032-ab313401--western-blot-img417913.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401)")
![Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401), expandable thumbnail](https://content.abcam.com/products/images/il-10-antibody-rp23040032-ab313401--western-blot-img417912.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Recombinant Fragment Protein within Mouse Il10. Database link P18893
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Mouse | Tested | Expected |
| Recombinant full length protein | Not recommended | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info 2.00000-2.50000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584).
Alternative names
Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF
Recommended products
Rabbit Recombinant Multiclonal IL-10 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Recombinant full length protein samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Il10. Database link P18893
- Clone number
- RP23040032
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-10 Antibody [RP23040032] (ab313401), expandable thumbnail](https://content.abcam.com/products/images/il-10-antibody-rp23040032-ab313401--immunocytochemistry-immunofluorescence-img417971.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-10 Antibody [RP23040032] (ab313401)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-10 Antibody [RP23040032] (ab313401)
Immunofluorescent analysis of 70% confluent log phase RAW 264.7 cells treated with LPS (200 µg/mL, 20 hrs) followed by PTI treatment (1X, 4 hrs) fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature labeling IL-10 with ab313401 at 1/100 dilution followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 at 1/2000 dilution for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with DAPI. F-actin (Panel c: Red) was stained with Rhodamine Phalloidin at 1/300 dilution.
Panel d represents the merged image showing secreted localization.
Panel e represents untreated cells with lower expression levels of IL10.
Panel f represents control cells with no primary antibody to assess background.
The images were captured at 60X magnification.![Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401), expandable thumbnail](https://content.abcam.com/products/images/il-10-antibody-rp23040032-ab313401--western-blot-img417913.jpg "Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401)")
Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401)
All lanes: Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401) at 2 µg/mL
Lane 1: Human recombinant IL-10 at 250 ng
Lane 2: Mouse recombinant IL-10 at 250 ng
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/20000 dilution
Observed band size: 18 kDa
![Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401), expandable thumbnail](https://content.abcam.com/products/images/il-10-antibody-rp23040032-ab313401--western-blot-img417912.jpg "Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401)")
Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401)
All lanes: Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401) at 2.5 µg/mL
All lanes: recombinant mouse IL-10 protein
Developed using the ECL technique.
Observed band size: 18 kDa
Downloads
Product protocols
- Visit the general protocols
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