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AB313401

Anti-IL-10 antibody [RP23040032]

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(1 Publication)

Rabbit Recombinant Multiclonal IL-10 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Recombinant full length protein samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il10.

View Alternative Names

Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-10 Antibody [RP23040032] (AB313401)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IL-10 Antibody [RP23040032] (AB313401)

Immunofluorescent analysis of 70% confluent log phase RAW 264.7 cells treated with LPS (200 µg/mL, 20 hrs) followed by PTI treatment (1X, 4 hrs) fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature labeling IL-10 with ab313401 at 1/100 dilution followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 at 1/2000 dilution for 45 minutes at room temperature (Panel a : Green). Nuclei (Panel b : Blue) were stained with DAPI. F-actin (Panel c : Red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing secreted localization. Panel e represents untreated cells with lower expression levels of IL10. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401)
  • WB

Supplier Data

Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401)

All lanes:

Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401) at 2 µg/mL

Lane 1:

Human recombinant IL-10 at 250 ng

Lane 2:

Mouse recombinant IL-10 at 250 ng

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/20000 dilution

Observed band size: 18 kDa

false

Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401)
  • WB

Supplier Data

Western blot - Anti-IL-10 Antibody [RP23040032] (AB313401)

All lanes:

Western blot - Anti-IL-10 Antibody [RP23040032] (ab313401) at 2.5 µg/mL

All lanes:

recombinant mouse IL-10 protein

Observed band size: 18 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040032

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Il10.

P18893

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-2.5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).
See full target information Il10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:5759-5775 PubMed40322534

2025

IGF1R Promotes Th17/Treg Cell Development in Experimental Autoimmune Prostatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Guan,Shaoyu Yue,Yiding Chen,Andong Cheng,Hao Li,Feixiang Yang,Yi Liu,Chaozhao Liang,Jialin Meng
View all publications
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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