Rabbit Recombinant Monoclonal IL-10RA antibody. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (AB323700), expandable thumbnail](https://content.abcam.com/products/images/il-10ra-antibody-epr24642-547-ab323700--flow-cytometry-img445131.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (AB323700)")
![Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (AB323700), expandable thumbnail](https://content.abcam.com/products/images/il-10ra-antibody-epr24642-547-ab323700--flow-cytometry-img445130.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (AB323700)")
![Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (AB323700), expandable thumbnail](https://content.abcam.com/products/images/il-10ra-antibody-epr24642-547-ab323700--flow-cytometry-img445129.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (AB323700)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-P | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Isotype Control
Target data
Function
Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683).
Alternative names
CD210, IL10R, IL10RA, Interleukin-10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1
Rabbit Recombinant Monoclonal IL-10RA antibody. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24642-547
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700), expandable thumbnail](https://content.abcam.com/products/images/il-10ra-antibody-epr24642-547-ab323700--flow-cytometry-img445131.jpg "Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700)")
Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700)
IL-10RA Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) cells using rabbit Anti-IL-10RA antibody
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling IL-10RA with ab323700 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
human PBMC are co-stained with CD56 conjugated BV421.
Gated on viable lymphocyte.![Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700), expandable thumbnail](https://content.abcam.com/products/images/il-10ra-antibody-epr24642-547-ab323700--flow-cytometry-img445130.jpg "Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700)")
Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700)
IL-10RA Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) cells using rabbit Anti-IL-10RA antibody
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling IL-10RA with ab323700 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
human PBMC are co-stained with CD4 conjugated BV421.
Gated on viable lymphocyte.![Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700), expandable thumbnail](https://content.abcam.com/products/images/il-10ra-antibody-epr24642-547-ab323700--flow-cytometry-img445129.jpg "Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700)")
Flow Cytometry - Anti-IL-10RA antibody [EPR24642-547] (ab323700)
IL-10RA Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-IL-10RA antibody
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling IL-10RA with ab323700 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
human PBMC are co-stained with CD14 conjugated Alexa Fluor®647.
Gated on viable cell.
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