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AB280572

Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free

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Rabbit Recombinant Monoclonal IL-10RB/IL-10R2 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CDw210b, CRFB4, D21S58, D21S66, IL10RB, Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, CRF2-4, IL-10R subunit 2, IL-10R2

4 Images
Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)

This data was developed using ab273038, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD14 conjugated to BV510. Gated on viable cells.

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)

This data was developed using ab273038, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)

This data was developed using ab273038, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD56 conjugated to PE. Gated on viable NK cells.

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] - BSA and Azide free (AB280572)

This data was developed using ab273038, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD14 conjugated to BV510. Gated on viable T cells and monocytes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24691-11

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab280572 is the carrier-free version of ab273038.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Shared cell surface receptor required for the activation of five class 2 cytokines : IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
See full target information IL10RB
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com