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AB89884

Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24]

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(1 Publication)

Mouse Monoclonal IL-10RB/IL-10R2 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IL10RB.

View Alternative Names

CDw210b, CRFB4, D21S58, D21S66, IL10RB, Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, CRF2-4, IL-10R subunit 2, IL-10R2

2 Images
Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (AB89884)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (AB89884)

Overlay histogram showing Jurkat cells stained with ab89884 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89884, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Western blot - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (AB89884)
  • WB

Supplier Data

Western blot - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (AB89884)

All lanes:

Western blot - Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (ab89884)

All lanes:

Human Placenta Tissue Lysate

Predicted band size: 36 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0360-8R24

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human IL10RB. The exact immunogen used to generate this antibody is proprietary information.

Q08334

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Primary Antibodies

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Primary Antibodies

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Anti-Progranulin antibody [EPR15864]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Shared cell surface receptor required for the activation of five class 2 cytokines : IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
See full target information IL10RB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 162:974-86 PubMed26317466

2015

Inhibiting DNA Methylation Causes an Interferon Response in Cancer via dsRNA Including Endogenous Retroviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine B Chiappinelli,Pamela L Strissel,Alexis Desrichard,Huili Li,Christine Henke,Benjamin Akman,Alexander Hein,Neal S Rote,Leslie M Cope,Alexandra Snyder,Vladimir Makarov,Sadna Budhu,Sadna Buhu,Dennis J Slamon,Jedd D Wolchok,Drew M Pardoll,Matthias W Beckmann,Cynthia A Zahnow,Taha Merghoub,Taha Mergoub,Timothy A Chan,Stephen B Baylin,Reiner Strick
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com