Anti-IL-11RA antibody [EPR5446] - BSA and Azide free

8 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling IL-11RA with Purified Anti-IL-11RA antibody [EPR5446] ab125015 at 1:250 dilution (3.2 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-11RA antibody [EPR5446] ab125015).

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  Immunocytochemistry/ Immunofluorescence - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling IL-11RA with Purified Anti-IL-11RA antibody [EPR5446] ab125015 at 1:100 dilution (8.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-11RA antibody [EPR5446] ab125015).

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  Western blot - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  This data was developed using Anti-IL-11RA antibody [EPR5446] ab125015, the same antibody clone in a different buffer formulation.
  

  The expression pattern is consistent with what has been described in the literature (PMID: 26876177).

  Blocking Buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-IL-11RA antibody [EPR5446] (Anti-IL-11RA antibody [EPR5446] ab125015) at 1/1000 dilution

  Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

  Lane 2: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg

  Lane 3: M1 (mouse myeloid leukemia myeloblast) whole cell lysate at 20 µg

  Lane 4: Mouse spleen tissue lysate at 20 µg

  Lane 5: Mouse testis tissue lysate at 20 µg

  Lane 6: Mouse thymus tissue lysate at 20 µg

  Lane 7: Mouse lymph node tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 45 kDa

  Observed band size: 42 kDa, 45 kDa

  Exposure time: 3min

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  Flow Cytometry (Intracellular) - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling IL-11RA with Purified Anti-IL-11RA antibody [EPR5446] ab125015 at 1/80 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  

  This data was developed using Anti-IL-11RA antibody [EPR5446] ab125015, the same antibody clone in a different buffer formulation.

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  Indirect ELISA - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  This data was developed using Anti-IL-11RA antibody [EPR5446] ab125015, the same antibody clone in a different buffer formulation.ELISA analysis of Human IL-11RA recombinant protein at 1000 ng/mL with Anti-IL-11RA antibody [EPR5446] ab125015. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  Overlay histogram showing K562 cells stained with unpurified Anti-IL-11RA antibody [EPR5446] ab125015 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-IL-11RA antibody [EPR5446] ab125015, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-11RA antibody [EPR5446] ab125015).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  Unpurified Anti-IL-11RA antibody [EPR5446] ab125015 at 1/500 staining IL11RA in paraffin embedded Human Kidney tissue by immunohistochemistry.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-11RA antibody [EPR5446] ab125015).

  Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

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  OI-RD Scanning - Anti-IL-11RA antibody [EPR5446] - BSA and Azide free (ab240950)

  We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
  Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.