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AB9992

Anti-IL-12 p70 antibody

4

(1 Review)

|

(9 Publications)

Goat Polyclonal IL-12 p70 antibody. Suitable for ELISA, WB, sELISA, IHC-P, FuncS (Neut/Block) and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IL12A.

View Alternative Names

NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

WB, FuncS (Neut/Block), IHC-P, ELISA, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human IL12B. Database link P29460

Reactivity data

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Product details

Although some customers have been successful in IHC we no longer batch test in this application.

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed : 8605935, PubMed : 8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 1673147, PubMed : 1674604, PubMed : 8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed : 8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed : 7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed : 7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed : 22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed : 22306691).
See full target information IL12A

Additional targets

IL12B

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Parasite immunology 45:e12971 PubMed36695719

2023

Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen M Sandoval Pacheco,Gabriela V Araujo Flores,Aurea F Ferreira,Vânia L R da Matta,Claudia M de Castro Gomes,Wilfredo H Sosa-Ochoa,Concepción Zúniga,Fernando T Silveira,Carlos E P Corbett,Márcia D Laurenti

Signal transduction and targeted therapy 8:24 PubMed36609561

2023

COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Pei Zhang,Zhi-Cheng He,Xiao-Hong Yao,Rui Tang,Jie Ma,Tao Luo,Chuhong Zhu,Tian-Ran Li,Xindong Liu,Dingyu Zhang,Shuyang Zhang,Yi-Fang Ping,Ling Leng,Xiu-Wu Bian

Frontiers in immunology 13:940645 PubMed35990663

2022

Single-cell RNA sequencing reveals distinct immunology profiles in human keloid.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Feng,Mengjie Shan,Yijun Xia,Zhi Zheng,Kai He,Yingxin Wei,Kexin Song,Tian Meng,Hao Liu,Yan Hao,Zhengyun Liang,Youbin Wang,Yongsheng Huang

Neuropathology : official journal of the Japanese Society of Neuropathology 42:181-189 PubMed35434787

2022

NLRP3 inflammasome inhibitor ameliorates ischemic stroke by reprogramming the phenotype of microglia/macrophage in a murine model of distal middle cerebral artery occlusion.

Applications

Unspecified application

Species

Unspecified reactive species

Hongning Jia,Xiaoyuan Qi,Lan Fu,Huijun Wu,Jinxing Shang,Mingwei Qu,Chaoping Yang,Jianping Wang

Molecular medicine reports 18:3537-3543 PubMed30066937

2018

Effects of aloe polysaccharide, a polysaccharide extracted from Aloe vera, on TNF‑α‑induced HaCaT cell proliferation and the underlying mechanism in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Leng,Li Pu,Longjiang Xu,Xin Shi,Jiang Ji,Kun Chen

International journal of oncology 50:545-554 PubMed28101568

2017

Puerarin inhibits M2 polarization and metastasis of tumor-associated macrophages from NSCLC xenograft model via inactivating MEK/ERK 1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Honggang Kang,Jing Zhang,Baozhong Wang,Meirong Liu,Jun Zhao,Mengxiang Yang,Yingxue Li

European journal of immunology 45:1772-82 PubMed25778793

2015

The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Céline Cousin,Aude Aubatin,Sabine Le Gouvello,Lionel Apetoh,Flavia Castellano,Valérie Molinier-Frenkel

PloS one 8:e75192 PubMed24069393

2013

Gastric epithelial expression of IL-12 cytokine family in Helicobacter pylori infection in human: is it head or tail of the coin?

Applications

IHC-P

Species

Human

Fadi Al-Sammak,Thomas Kalinski,Sönke Weinert,Alexander Link,Thomas Wex,Peter Malfertheiner

PloS one 3:e1999 PubMed18431484

2008

Anti-HIV activity mediated by natural killer and CD8+ cells after toll-like receptor 7/8 triggering.

Applications

Neut

Species

Human

Erika Schlaepfer,Roberto F Speck
View all publications

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