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AB80682

Anti-IL-12 antibody [AP-MAB0853]

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(3 Publications)

Rat Monoclonal IL-12A antibody. Carrier free. Suitable for IP, Flow Cyt, WB, FuncS (Neut/Block) and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Il12a.

View Alternative Names

Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

AP-MAB0853

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse

Applications

IP, WB, FuncS (Neut/Block), Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Mouse Il12a. The exact immunogen used to generate this antibody is proprietary information.

P43431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>Neutralizes the bioactivity of natural or recombinant IL12 both in vivo and in vitro.</p>" } } }
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Product details

Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).

ab80682 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 8766560). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (By similarity). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine (PubMed : 18033300, PubMed : 30197594). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (By similarity).
See full target information Il12a
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Hepatology (Baltimore, Md.) 77:874-887 PubMed35592906

2023

Myeloid p38 activation maintains macrophage-liver crosstalk and BAT thermogenesis through IL-12-FGF21 axis.

Applications

Unspecified application

Species

Unspecified reactive species

María Crespo,Ivana Nikolic,Alfonso Mora,Elena Rodríguez,Luis Leiva-Vega,Aránzazu Pintor-Chocano,Daniel Horrillo,Lourdes Hernández-Cosido,Jorge L Torres,Eva Novoa,Rubén Nogueiras,Gema Medina-Gómez,Miguel Marcos,Magdalena Leiva,Guadalupe Sabio

Infection and immunity 84:2802-12 PubMed27456834

2016

Proinflammatory M1 Macrophages Inhibit RANKL-Induced Osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuguno Yamaguchi,Alexandru Movila,Shinsuke Kataoka,Wichaya Wisitrasameewong,Montserrat Ruiz Torruella,Michiaki Murakoshi,Shinya Murakami,Toshihisa Kawai

Molecular medicine reports 12:7425-32 PubMed26459920

2015

Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Zhou,Xiaoyong Li,Meina Feng,Qi Zhang,Zhendong Yang
View all publications
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