Rabbit Recombinant Monoclonal IL-12B antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-IL-12 p40 antibody [EPR5738] (AB131156), expandable thumbnail](https://content.abcam.com/products/images/il-12-p40-antibody-epr5738-ab131156--western-blot-img81600.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-12 p40 antibody [EPR5738] (AB131156)")
![OI-RD Scanning - Anti-IL-12 p40 antibody [EPR5738] (AB131156), expandable thumbnail](https://content.abcam.com/products/images/il-12-p40-antibody-epr5738-ab131156--oi-rd-scanning-img137629.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-IL-12 p40 antibody [EPR5738] (AB131156)")
Publications
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- The Journal of pharmacology and experimental therapeutics 381:247-2562022Anisodamine Enhances Macrophage M2 Polarization through Suppressing G9a-Mediated Interferon Regulatory Factor 4 Silencing to Alleviate Lipopolysaccharide-Induced Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfeng Zhang,Dingli Song,Ziyang Peng,Rui Wang,Kai Li,Hong Ren,Xin Sun,Ning Du,Shou-Ching TangPubMed 35383125
- Frontiers in oncology 12:7799392022Potential Resistance to Antineoplastic Aminated Fullerenes Mediated by M2-Like Monocyte-Derived Exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiawei Huo,Wei Zhou,Yang Liu,Sifen Yang,Jie Li,Chunru WangPubMed 35433481
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50000 - 1/200000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Alternative names
NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40
Recommended products
Rabbit Recombinant Monoclonal IL-12B antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5738
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.26 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-IL-12 p40 antibody [EPR5738] (ab131156), expandable thumbnail](https://content.abcam.com/products/images/il-12-p40-antibody-epr5738-ab131156--western-blot-img81600.jpg "Western blot - Anti-IL-12 p40 antibody [EPR5738] (ab131156)")
Western blot - Anti-IL-12 p40 antibody [EPR5738] (ab131156)
All lanes: Western blot - Anti-IL-12 p40 antibody [EPR5738] (ab131156) at 1/50000 dilution
All lanes: Human IL-12 p40 recombinant protein at 0.01 µg
Predicted band size: 37 kDa
![OI-RD Scanning - Anti-IL-12 p40 antibody [EPR5738] (ab131156), expandable thumbnail](https://content.abcam.com/products/images/il-12-p40-antibody-epr5738-ab131156--oi-rd-scanning-img137629.jpg "OI-RD Scanning - Anti-IL-12 p40 antibody [EPR5738] (ab131156)")
OI-RD Scanning - Anti-IL-12 p40 antibody [EPR5738] (ab131156)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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