Anti-IL-12A antibody [EP5737]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (AB131039)

Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labelling IL-12A with unpurified ab131039 at dilution of 1 : 250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (AB131039)

Flow cytometry analysis of THP-1 cells treated with 100ng/ml PMA for 30min,then 1© : g/ml LPS for 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 © : g/ml) (Red). Cells were fixed with 4% paraformaldehyde . Dylight488 (ab98462) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). Untreated cells (Green)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (AB131039)

Formaldehyde-fixed, paraffin-embedded human colon tissue stained for IL-12A using ab131039 at 1/500 dilution in immunohistochemical analysis.

This image is courtesy of an anonymous Abreview.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (AB131039)

Immunohistochemical staining of paraffin embedded human gastric carcinoma tissue section labelling IL-12A with purified ab131039 at dilution of 1/800. The secondary antibody used was HRP conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (AB131039)

Flow cytometry analysis of Raw264.7 cells treated with 100ng/ml for LPS 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 © : g/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

• WB

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Western blot - Anti-IL-12A antibody [EP5737] (AB131039)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution

All lanes:

HuT-78 (human Sezary syndrome) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 12 kDa,25 kDa

Observed band size: 35 kDa

false

• WB

Unknown

Western blot - Anti-IL-12A antibody [EP5737] (AB131039)

All lanes:

Western blot - Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution

Lane 1:

NIH3T3 cell lysate at 10 µg

Lane 2:

HuT-78 cell lysate at 10 µg

Lane 3:

Raw 264.7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa

false

• WB

Unknown

Western blot - Anti-IL-12A antibody [EP5737] (AB131039)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-12A antibody [EP5737] (ab131039) at 1/5000 dilution

Lane 1:

NIH/3T3 (mouse embryo) whole cell lysate at 20 µg

Lane 2:

Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 35 kDa

false