Anti-IL-12A antibody [EP5737] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (AB214433)

Intracellular Flow Cytometry analysis of THP-1 cells treated with 100ng/ml PMA for 30min,then 1μg/ml LPS for 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02μg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Dylight488® (ab98462) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). Untreated cells (Green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

• IHC-P

AbReview52959****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (AB214433)

Formaldehyde-fixed, paraffin-embedded human colon tissue stained for IL12A using ab131039 at 1/500 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

This image is courtesy of an anonymous Abreview.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (AB214433)

Immunohistochemical staining of paraffin embedded human gastric carcinoma tissue section labelling IL12A with purified ab131039 at dilution of 1/800. The secondary antibody used was HRP conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (AB214433)

Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labelling IL12A with unpurified ab131039 at dilution of 1 : 250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (AB214433)

Intracellular Flow Cytometry analysis of Raw264.7 cells treated with 100ng/ml for LPS 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02μg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).