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AB133751

Anti-IL-12A antibody [EPR5736]

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(7 Publications)

Rabbit Recombinant Monoclonal IL-12A antibody. Suitable for WB and reacts with Mouse, Rat, Recombinant full length protein - Human, Human samples. Cited in 7 publications.

View Alternative Names

NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

3 Images
Western blot - Anti-IL-12A antibody [EPR5736] (AB133751)
  • WB

Lab

Western blot - Anti-IL-12A antibody [EPR5736] (AB133751)

All lanes:

Western blot - Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution

Lane 1:

HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysates at 20 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg

Lane 3:

TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 5:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Lane 6:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 7:

Rat spleen lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 35 kDa

false

Western blot - Anti-IL-12A antibody [EPR5736] (AB133751)
  • WB

Lab

Western blot - Anti-IL-12A antibody [EPR5736] (AB133751)

The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.

Lanes 1 - 3:

Western blot - Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-IL-12B antibody [EPR5739] (<a href='/en-us/products/primary-antibodies/il-12b-antibody-epr5739-ab133752'>ab133752</a>) at 1/1000 dilution

All lanes:

Human IL-12 recombinant protein at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 25 kDa,37 kDa

Observed band size: 29 kDa,40 kDa

false

OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (AB133751)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (AB133751)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-IL-12A antibody [EPR5736] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5736

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed : 8605935, PubMed : 8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 1673147, PubMed : 1674604, PubMed : 8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed : 8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed : 7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed : 7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed : 22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed : 22306691).
See full target information IL12A
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadg6874 PubMed37878703

2023

Human interleukin-12α and EBI3 are cytokines with anti-inflammatory functions.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Hildenbrand,Sina Bohnacker,Priyanka Rajeev Menon,Anna Kerle,Ulrich F Prodjinotho,Franziska Hartung,Patrick C Strasser,Dragana A M Catici,Florian Rührnößl,Martin Haslbeck,Kathrin Schumann,Stephanie I Müller,Clarissa Prazeres da Costa,Julia Esser-von Bieren,Matthias J Feige

The Journal of biological chemistry 298:102677 PubMed36336075

2022

A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yonatan G Mideksa,Isabel Aschenbrenner,Anja Fux,Dinah Kaylani,Caroline A M Weiß,Tuan-Anh Nguyen,Nina C Bach,Kathrin Lang,Stephan A Sieber,Matthias J Feige

eLife 11: PubMed35983991

2022

HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Jia Luo,Sung-Liang Yu,Chia-Ching Chang,Min-Hui Chien,Ya-Ling Chang,Keng-Mao Liao,Pei-Chun Lin,Kuei-Pin Chung,Ya-Hui Chuang,Jeremy J W Chen,Pan-Chyr Yang,Kang-Yi Su

Molecular immunology 126:120-128 PubMed32823236

2020

Influence of glycosylation on IL-12 family cytokine biogenesis and function.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Bohnacker,Karen Hildenbrand,Isabel Aschenbrenner,Stephanie I Müller,Julia Esser-von Bieren,Matthias J Feige

Experimental and therapeutic medicine 19:1695-1700 PubMed32104222

2020

Interleukin-35 reduces inflammation in acute lung injury through inhibiting TLR4/NF-κB signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Pan,Xiaoheng Xu,Yan Wang,Xingyu Song

Frontiers in immunology 10:787 PubMed31068929

2019

Silica Particles Mediate Phenotypic and Functional Alteration of Dendritic Cells and Induce Th2 Cell Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Suna Liu,Changfu Hao,Lei Bao,Dehua Zhao,Hongyi Zhang,Jianyong Hou,Di Wang,Huiting Chen,Feifei Feng,Wu Yao

The Journal of biological chemistry 292:8073-8081 PubMed28325840

2017

Assembly-induced folding regulates interleukin 12 biogenesis and secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Reitberger,Pascal Haimerl,Isabel Aschenbrenner,Julia Esser-von Bieren,Matthias J Feige
View all publications
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