Rabbit Recombinant Monoclonal IL-12A antibody. Suitable for WB and reacts with Mouse, Rat, Recombinant full length protein - Human, Human samples. Cited in 7 publications.
Images
![Western blot - Anti-IL-12A antibody [EPR5736] (AB133751), expandable thumbnail](https://content.abcam.com/products/images/il-12a-antibody-epr5736-ab133751--western-blot-img15782.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-12A antibody [EPR5736] (AB133751)")
![Western blot - Anti-IL-12A antibody [EPR5736] (AB133751), expandable thumbnail](https://content.abcam.com/products/images/il-12a-antibody-epr5736-ab133751--western-blot-img8659.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-12A antibody [EPR5736] (AB133751)")
![OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (AB133751), expandable thumbnail](https://content.abcam.com/products/images/il-12a-antibody-epr5736-ab133751--oi-rd-scanning-img83994.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (AB133751)")
Publications
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- Science advances 9:eadg68742023Human interleukin-12α and EBI3 are cytokines with anti-inflammatory functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren Hildenbrand,Sina Bohnacker,Priyanka Rajeev Menon,Anna Kerle,Ulrich F Prodjinotho,Franziska Hartung,Patrick C Strasser,Dragana A M Catici,Florian Rührnößl,Martin Haslbeck,Kathrin Schumann,Stephanie I Müller,Clarissa Prazeres da Costa,Julia Esser-von Bieren,Matthias J FeigePubMed 37878703
- The Journal of biological chemistry 298:1026772022A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonatan G Mideksa,Isabel Aschenbrenner,Anja Fux,Dinah Kaylani,Caroline A M Weiß,Tuan-Anh Nguyen,Nina C Bach,Kathrin Lang,Stephan A Sieber,Matthias J FeigePubMed 36336075
- eLife 11:2022HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Jia Luo,Sung-Liang Yu,Chia-Ching Chang,Min-Hui Chien,Ya-Ling Chang,Keng-Mao Liao,Pei-Chun Lin,Kuei-Pin Chung,Ya-Hui Chuang,Jeremy J W Chen,Pan-Chyr Yang,Kang-Yi SuPubMed 35983991
- Molecular immunology 126:120-1282020Influence of glycosylation on IL-12 family cytokine biogenesis and function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSina Bohnacker,Karen Hildenbrand,Isabel Aschenbrenner,Stephanie I Müller,Julia Esser-von Bieren,Matthias J FeigePubMed 32823236
- Experimental and therapeutic medicine 19:1695-17002020Interleukin-35 reduces inflammation in acute lung injury through inhibiting TLR4/NF-κB signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Pan et. al.PubMed 32104222
- Frontiers in immunology 10:7872019Silica Particles Mediate Phenotypic and Functional Alteration of Dendritic Cells and Induce Th2 Cell Polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuna Liu et. al.PubMed 31068929
- The Journal of biological chemistry 292:8073-80812017Assembly-induced folding regulates interleukin 12 biogenesis and secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusanne Reitberger et. al.PubMed 28325840
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Not recommended | Tested |
| Mouse | Not recommended | Tested |
| Rat | Not recommended | Tested |
| Recombinant full length protein - Human | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed:8605935, PubMed:8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed:1673147, PubMed:1674604, PubMed:8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed:8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed:7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed:7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed:22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed:22306691).
Alternative names
NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35
Recommended products
Rabbit Recombinant Monoclonal IL-12A antibody. Suitable for WB and reacts with Mouse, Rat, Recombinant full length protein - Human, Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5736
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 5.5 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-IL-12A antibody [EPR5736] (ab133751), expandable thumbnail](https://content.abcam.com/products/images/il-12a-antibody-epr5736-ab133751--western-blot-img15782.jpg "Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)")
Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)
The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.
Lanes 1 - 3: Western blot - Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution
Lanes 1 - 3: Western blot - Anti-IL-12B antibody [EPR5739] (Anti-IL-12B antibody [EPR5739] ab133752) at 1/1000 dilution
All lanes: Human IL-12 recombinant protein at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 25 kDa, 37 kDa
Observed band size: 29 kDa, 40 kDa
![Western blot - Anti-IL-12A antibody [EPR5736] (ab133751), expandable thumbnail](https://content.abcam.com/products/images/il-12a-antibody-epr5736-ab133751--western-blot-img8659.jpg "Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)")
Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)
All lanes: Western blot - Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution
Lane 1: HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysates at 20 µg
Lane 2: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg
Lane 3: TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 5: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
Lane 7: Rat spleen lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 35 kDa
![OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (ab133751), expandable thumbnail](https://content.abcam.com/products/images/il-12a-antibody-epr5736-ab133751--oi-rd-scanning-img83994.jpg "OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (ab133751)")
OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (ab133751)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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