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AB244924

Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector)

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Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (ab244924) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting IL-12B in ELISA. Suitable for Human, Monkey.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40

3 Images
Sandwich ELISA - Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (AB244924)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (AB244924)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab220656).

Sandwich ELISA - Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (AB244924)
  • sELISA

Lab

Sandwich ELISA - Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (AB244924)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab223592).

Sandwich ELISA - Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (AB244924)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (AB244924)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab314605), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20096-13

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Monkey

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-IL-12B antibody [EPR20096-13] - BSA and Azide free (Detector) (ab244924) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human, Monkey samples.

Other related products
We have a range of other formats of antibody clone [EPR20096-13] also available for your convenience: ab133752, Alexa Fluor® 488 - ab214253, Carrier free - ab236059, Carrier free - ab246704, Alexa Fluor® 647 - ab305042, Carrier free - ab244924

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
See full target information IL12B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com