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Rabbit Recombinant Monoclonal IL-12B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Human samples. Cited in 6 publications.

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Images

Western blot - Anti-IL-12B antibody [EPR5739] (AB133752), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (AB133752), expandable thumbnail
  • Western blot - Anti-IL-12B antibody [EPR5739] (AB133752), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] (AB133752), expandable thumbnail
  • OI-RD Scanning - Anti-IL-12B antibody [EPR5739] (AB133752), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIPICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Tested
Tested
Recombinant full length protein - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

1/1000 - 1/10000

Notes

This antibody is unsuitable to detect endogenous IL12B in WB

Species

Human

Dilution info

1/1000 - 1/10000

Notes

This antibody is unsuitable to detect endogenous IL12B in WB

Not recommended
Not recommended

Species

Human, Recombinant full length protein - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/300

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal IL-12B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Human samples. Cited in 6 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR5739

Purification technique

Affinity purification Protein A

Specificity

This antibody is unsuitable to detect endogenous IL-12B in WB.

Dissociation constant

4.3 x 10-10 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-IL-12B antibody [EPR5739] (ab133752), expandable thumbnail

    Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)

    The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.

    Lanes 1 - 3: Western blot - Anti-IL-12A antibody [EPR5736] (Anti-IL-12A antibody [EPR5736] ab133751) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution

    All lanes: Human IL-12 recombinant protein at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa, 37 kDa

    Observed band size: 29 kDa, 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (ab133752), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (ab133752)

    Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling IL-12B with Purified ab133752 at 1:100 dilution (3.3 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were not counterstained. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-IL-12B antibody [EPR5739] (ab133752), expandable thumbnail

    Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)

    This image was generated using the unpurified version of the product.

    All lanes: Western blot - Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution

    All lanes: Human IL-12B recombinant protein at 0.01 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 37 kDa

    Observed band size: 40 kDa

  • Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] (ab133752), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] (ab133752)

    Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling IL-12B (red) with ab133752 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • OI-RD Scanning - Anti-IL-12B antibody [EPR5739] (ab133752), expandable thumbnail

    OI-RD Scanning - Anti-IL-12B antibody [EPR5739] (ab133752)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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