Rabbit Recombinant Monoclonal IL-12B antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (AB246704), expandable thumbnail](https://content.abcam.com/products/images/il-12b-antibody-epr5739-low-endotoxin-azide-free-ab246704--immunocytochemistry-immunofluorescence-img57416.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (AB246704)")
![Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (AB246704), expandable thumbnail](https://content.abcam.com/products/images/il-12b-antibody-epr5739-low-endotoxin-azide-free-ab246704--flow-cytometry-intracellular-img136916.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (AB246704)")
![OI-RD Scanning - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (AB246704), expandable thumbnail](https://content.abcam.com/products/images/il-12b-antibody-epr5739-low-endotoxin-azide-free-ab246704--oi-rd-scanning-img85014.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (AB246704)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
Target data
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Alternative names
NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40
Recommended products
Rabbit Recombinant Monoclonal IL-12B antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5739
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is unsuitable to detect endogenous IL-12B in WB.
- Dissociation constant
- 4.3 x 10-10 M
- Concentration
- Purification notes
Endotoxin level is less than 1 EU/ml as determined by the TAL test.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab246704 is the carrier-free version of Anti-IL-12B antibody [EPR5739] ab133752.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704), expandable thumbnail](https://content.abcam.com/products/images/il-12b-antibody-epr5739-low-endotoxin-azide-free-ab246704--immunocytochemistry-immunofluorescence-img57416.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704)
Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling IL-12B with Purified Anti-IL-12B antibody [EPR5739] ab133752 at 1:100 dilution (3.3 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were not counterstained. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-IL-12B antibody [EPR5739] ab133752).
![Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704), expandable thumbnail](https://content.abcam.com/products/images/il-12b-antibody-epr5739-low-endotoxin-azide-free-ab246704--flow-cytometry-intracellular-img136916.jpg "Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704)")
Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling IL-12B (red) with Anti-IL-12B antibody [EPR5739] ab133752 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-IL-12B antibody [EPR5739] ab133752).
![OI-RD Scanning - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704), expandable thumbnail](https://content.abcam.com/products/images/il-12b-antibody-epr5739-low-endotoxin-azide-free-ab246704--oi-rd-scanning-img85014.jpg "OI-RD Scanning - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704)")
OI-RD Scanning - Anti-IL-12B antibody [EPR5739] - Low endotoxin, Azide free (ab246704)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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