Rabbit Recombinant Monoclonal IL-12RB1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16850.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892)")
![Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-frozen-sections-img6932.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102882.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892)")
![Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-frozen-sections-img121286.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96305.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (AB280892)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Expected |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
Alternative names
CD212, IL12R, IL12RB, IL12RB1, Interleukin-12 receptor subunit beta-1, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, IL-12R-beta-1, IL-12RB1, IL-12 receptor beta component
Recommended products
Rabbit Recombinant Monoclonal IL-12RB1 antibody. Carrier free. Suitable for IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23693-37
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab280892 is the carrier-free version of Anti-IL-12RB1 antibody [EPR23693-37] ab256805.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16850.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
This data was developed using Anti-IL-12RB1 antibody [EPR23693-37] ab256805, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IL-12RB1 with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes of human colon. The section was incubated with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-frozen-sections-img6932.jpg "Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
This data was developed using Anti-IL-12RB1 antibody [EPR23693-37] ab256805, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Perfused-fixed mouse lymph node tissue labeling IL-12RB1 with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 at 1/50 (11.38 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lymph node. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102882.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
This data was developed using Anti-IL-12RB1 antibody [EPR23693-37] ab256805, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IL-12RB1 with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil (PMID: 11120812). The section was incubated with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-frozen-sections-img121286.jpg "Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
Immunohistochemistry (Frozen sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
This data was developed using Anti-IL-12RB1 antibody [EPR23693-37] ab256805, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Perfused-fixed mouse colon tissue labeling IL-12RB1 with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 at 1/50 (11.38 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on lymphocytes of mouse colon. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96305.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
This data was developed using Anti-IL-12RB1 antibody [EPR23693-37] ab256805, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma tissue labelling IL-12RB1 with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human diffuse large B-cell lymphoma. The section was incubated with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34514.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
This data was developed using Anti-IL-12RB1 antibody [EPR23693-37] ab256805, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling IL-12RB1 with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes of mouse colon. The section was incubated with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28157.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)
This data was developed using Anti-IL-12RB1 antibody [EPR23693-37] ab256805, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling IL-12RB1 with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 at 1/200 (2.845 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on lymphocytes of rat colon. The section was incubated with Anti-IL-12RB1 antibody [EPR23693-37] ab256805 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34514.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892), expandable thumbnail](https://content.abcam.com/products/images/il-12rb1-antibody-epr23693-37-bsa-and-azide-free-ab280892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28157.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12RB1 antibody [EPR23693-37] - BSA and Azide free (ab280892)")
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