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AB325325

Anti-IL-13 antibody [EPR27282-114]

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Rabbit Recombinant Monoclonal IL-13 antibody. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Mouse samples.

View Alternative Names

Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600

3 Images
Flow Cytometry (Intracellular) - Anti-IL-13 antibody [EPR27282-114] (AB325325)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IL-13 antibody [EPR27282-114] (AB325325)

Flow cytometric analysis of Enriched mouse CD4+ T cells were cultured under Th2-polarizing conditions for 4 days then treated with 10ng/mL PMA, 500ng/mL Ionomycin and 300ng/ml BFA for 4 hours (Lower left and right) Enriched mouse CD4+ T cells were cultured under Th2-polarizing conditions for 4 days (Upper left and right) cells labelling IL-13 with abNeed ID4646 at 1/5000 dilution (0.01ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-GATA-3 antibody conjugated to Alexa Fluor®488.

Immunocytochemistry/ Immunofluorescence - Anti-IL-13 antibody [EPR27282-114] (AB325325)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-13 antibody [EPR27282-114] (AB325325)

Confocal image showing cytoplasmic staining in subsets of mouse Th2-polarized CD4+ naïve T cells 4 days activated with PMA (10ng/mL), Ionomycin (500ng/mL) and BFA (300ng/ml) for 4 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Th2 unstimulated : Mouse Th2-polarized CD4+ naïve T cells for 4 days.

Th2 stimulated : Mouse Th2-polarized CD4+ naïve T cells 4 days activated with PMA (10ng/mL), Ionomycin (500ng/mL) and BFA (300ng/ml) for 4 hours.

Th1 unstimulated : Mouse Th1-polarized CD4+ naïve T cells for 4 days.

Th1 stimulated : Mouse Th1-polarized CD4+ naïve T cells for 4 days activated with PMA (10ng/mL), Ionomycin (500ng/mL) and BFA (300ng/ml) for 4 hours.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Anti-IL-13 antibody [EPR27282-114] (AB325325)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IL-13 antibody [EPR27282-114] (AB325325)

Flow cytometric analysis of Enriched mouse CD4+ T cells were cultured under Th1-polarizing conditions for 4 days then treated with 10ng/mL PMA, 500ng/mL Ionomycin and 300ng/ml BFA for 4 hours (Lower left and right); Enriched mouse CD4+ T cells were cultured under Th1-polarizing conditions for 4 days (Upper left and right) cells labelling IL-13 with ab325325 at 1/5000 dilution (0.01ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with anti-IFN-g antibody conjugated to APC.

  • Carrier free

    Anti-IL-13 antibody [EPR27282-114] – BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27282-114

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 15361238). Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (By similarity). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (PubMed : 34789557). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 34795444, PubMed : 8871614). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 29305434).
See full target information Il13
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com