Anti-IL-13 antibody [EPR27282-114] – BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IL-13 antibody [EPR27282-114] – BSA and Azide free (AB325329)

This data was developed using ab325325, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Enriched mouse CD4+ T cells were cultured under Th2-polarizing conditions for 4 days then treated with 10ng/mL PMA, 500ng/mL Ionomycin and 300ng/ml BFA for 4 hours (Lower left and right) Enriched mouse CD4+ T cells were cultured under Th2-polarizing conditions for 4 days (Upper left and right) cells labelling IL-13 with abNeed ID4646 at 1/5000 dilution (0.01ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-GATA-3 antibody conjugated to Alexa Fluor®488.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-13 antibody [EPR27282-114] – BSA and Azide free (AB325329)

This data was developed using ab325325, the same antibody clone in a different buffer formulation.

Confocal image showing cytoplasmic staining in subsets of mouse Th2-polarized CD4+ naïve T cells 4 days activated with PMA (10ng/mL), Ionomycin (500ng/mL) and BFA (300ng/ml) for 4 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Th2 unstimulated : Mouse Th2-polarized CD4+ naïve T cells for 4 days.

Th2 stimulated : Mouse Th2-polarized CD4+ naïve T cells 4 days activated with PMA (10ng/mL), Ionomycin (500ng/mL) and BFA (300ng/ml) for 4 hours.

Th1 unstimulated : Mouse Th1-polarized CD4+ naïve T cells for 4 days.

Th1 stimulated : Mouse Th1-polarized CD4+ naïve T cells for 4 days activated with PMA (10ng/mL), Ionomycin (500ng/mL) and BFA (300ng/ml) for 4 hours.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IL-13 antibody [EPR27282-114] – BSA and Azide free (AB325329)

This data was developed using ab325325, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Enriched mouse CD4+ T cells were cultured under Th1-polarizing conditions for 4 days then treated with 10ng/mL PMA, 500ng/mL Ionomycin and 300ng/ml BFA for 4 hours (Lower left and right); Enriched mouse CD4+ T cells were cultured under Th1-polarizing conditions for 4 days (Upper left and right) cells labelling IL-13 with ab325325 at 1/5000 dilution (0.01ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with anti-IFN-g antibody conjugated to APC.