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AB322050

Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] - BSA and Azide free

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Rabbit Recombinant Monoclonal IL-13 receptor alpha 1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CD213a1, IL13R, IL13RA, IL13RA1, Interleukin-13 receptor subunit alpha-1, IL-13 receptor subunit alpha-1, IL-13R subunit alpha-1, IL-13R-alpha-1, IL-13RA1, Cancer/testis antigen 19, CT19

2 Images
Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] - BSA and Azide free (AB322050)
  • WB

Supplier Data

Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] - BSA and Azide free (AB322050)

This data was developed using ab322049, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

IL-13 receptor alpha 1 is a glycoprotein of approximately 70 kDa and detected as a 49kDa band after treated with Protein Deglycosylation MIX II.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] (<a href='/en-us/products/primary-antibodies/il-13-receptor-alpha-1-antibody-epr28795-26-ab322049'>ab322049</a>) at 1/1000 dilution

Lane 1:

Untreated HDLM-2 (human hodgkin lymphoma cell) whole cell lysate at 20 µg

Lane 2:

HDLM-2 whole cell lysate treated with Protein Deglycosylation Mix II at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,49 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] - BSA and Azide free (AB322050)
  • WB

Supplier Data

Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] - BSA and Azide free (AB322050)

This data was developed using ab322049, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Jurkat (PMID : 15163701) Raji Ramos (PMID : 11591117).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL-13 receptor alpha 1 antibody [EPR28795-26] (<a href='/en-us/products/primary-antibodies/il-13-receptor-alpha-1-antibody-epr28795-26-ab322049'>ab322049</a>) at 1/1000 dilution

Lane 1:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 30 µg

Lane 3:

Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate at 30 µg

Lane 4:

Ramos (human burkitt's lymphoma b lymphocyte) whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,36 kDa

false

Exposure time: 114s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28795-26

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab322050 is the carrier-free version of ab322049.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
See full target information IL13RA1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com