Rabbit Recombinant Monoclonal IL-13 receptor alpha 2 antibody. Carrier free. Suitable for IP, I-ELISA, WB, ICC/IF and reacts with Human, Recombinant full length protein samples.
Images
![Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--western-blot-img6612.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--immunocytochemistry-immunofluorescence-img126901.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874)")
![Immunoprecipitation - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--immunoprecipitation-img3595.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--immunocytochemistry-immunofluorescence-img109940.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874)")
![Indirect ELISA - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--indirect-elisa-img415808.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (AB263874)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | IP | I-ELISA | WB | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Recombinant full length protein | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Recombinant full length protein | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed:11861389, PubMed:17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed:11861389, PubMed:17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed:16327802).
Alternative names
CD213a2, IL13R, IL13RA2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Interleukin-13-binding protein
Recommended products
Rabbit Recombinant Monoclonal IL-13 receptor alpha 2 antibody. Carrier free. Suitable for IP, I-ELISA, WB, ICC/IF and reacts with Human, Recombinant full length protein samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22978-163
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab263874 is the carrier-free version of Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--western-blot-img6612.jpg "Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)")
Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)
This data was developed using the same antibody clone in a different buffer formulation (Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 observed at 49 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 was shown to react with IL-13 receptor alpha 2 in wild-type A375 cells in western blot with loss of signal observed in IL13RA2 knockout cell line ab273371 (knockout cell lysate Human IL13RA2 knockout A375 cell lysate ab275532). Wild-type and IL13RA2 knockout A375 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] (Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044) at 1/1000 dilution
Lane 1: Wild-type A375 cell lysate at 30 µg
Lane 2: IL13RA2 knockout A375 cell lysate at 30 µg
Lane 2: Western blot - Human IL13RA2 knockout A375 cell line (Human IL13RA2 knockout A375 cell line ab273381)
Lane 3: U-87-MG cell lysate at 30 µg
Lane 4: Daudi cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 49 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--immunocytochemistry-immunofluorescence-img126901.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)
This data was developed using the same antibody clone in a different buffer formulation (Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044). Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 staining IL-13 receptor alpha 2 in wild-type A375 cells (top panel) and IL13RA2 knockout A375 cells (bottom panel) (Human IL13RA2 knockout A375 cell line ab273381). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 at 1/500 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).![Immunoprecipitation - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--immunoprecipitation-img3595.jpg "Immunoprecipitation - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)")
Immunoprecipitation - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)
IL-13 receptor alpha 2 was immunoprecipitated from 0.35 mg A375 (Human malignant melanoma epithelial cell) whole cell lysate 10μg with Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: A375 (Human malignant melanoma epithelial cell) whole cell lysate 10μg
Lane 2: Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 IP in A375 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 in A375 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044).
All lanes: Immunoprecipitation - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] (Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044)
Predicted band size: 44 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--immunocytochemistry-immunofluorescence-img109940.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labeling IL-13 receptor alpha 2 with Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in A375 cell line.
Negative control: Daudi (PMID: 26059190.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044).
![Indirect ELISA - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874), expandable thumbnail](https://content.abcam.com/products/images/il-13-receptor-alpha-2-antibody-epr22978-163-bsa-and-azide-free-ab263874--indirect-elisa-img415808.jpg "Indirect ELISA - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)")
Indirect ELISA - Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] - BSA and Azide free (ab263874)
This data was developed using Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044, the same antibody clone in a different buffer formulation.ELISA analysis of IL13RA2 recombinant protein at 1000 ng/mL with Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] ab260044. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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