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AB324048

Anti-IL-13 receptor alpha 2 antibody [EPR29921-581]

  • RabMAb
  • Recombinant
  • 20ul selling size
  • KO Validated
  • What is this?

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Rabbit Recombinant Monoclonal IL-13 receptor alpha 2 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

View Alternative Names

CD213a2, IL13R, IL13RA2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Interleukin-13-binding protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Wild-type A375 cells and IL13RA2 KO A375 cells labelling IL-13 receptor alpha 2 with ab324048 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) antibody at 1/1000 dilution (Green).

Confocal image showing membranous and cytoplasmic staining in parental A375 cell line (shown in green).

The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-alpha Tubulin mouse monoclonal antibody (ab7291) was used to counterstain tubulin at 1/1000 dilution (Magenta).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Flow cytometric analysis of A-172 (human brain glioblastoma cell, Right) / Daudi (human Burkitt's lymphoma lymphoblast, Left) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : Daudi.
Gated on viable cells.

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Flow cytometric analysis of IL13RA2 KO A375 (IL-13 receptor alpha 2 knock out human malignant melanoma epithelial cell, Magenta) / A375 (Green) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29921-581

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB274400

Human IL-13R alpha 2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-13R alpha 2 ELISA Kit (AB274400)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed : 11861389, PubMed : 17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed : 11861389, PubMed : 17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed : 16327802).
See full target information IL13RA2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com