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Rabbit Recombinant Monoclonal IL-13 receptor alpha 2 antibody. Suitable for Flow Cyt and reacts with Human samples.

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Images

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048), expandable thumbnail
  • Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Target data

Function

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed:11861389, PubMed:17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed:11861389, PubMed:17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed:16327802).

Alternative names

Rabbit Recombinant Monoclonal IL-13 receptor alpha 2 antibody. Suitable for Flow Cyt and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29921-581
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (ab324048), expandable thumbnail

    Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (ab324048)

    IL-13 receptor alpha 2 Flow Cytometry staining using rabbit Anti-IL-13 receptor alpha 2 antibody

    Flow cytometric analysis of IL13RA2 KO A375 (IL-13 receptor alpha 2 knock out human malignant melanoma epithelial cell, Magenta) / A375 (Green) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Magenta and Green compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and Grey isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

    Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (ab324048), expandable thumbnail

    Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (ab324048)

    IL-13 receptor alpha 2 Flow Cytometry staining using rabbit Anti-IL-13 receptor alpha 2 antibody

    Flow cytometric analysis of A-172 (human brain glioblastoma cell, Right) / Daudi (human Burkitt's lymphoma lymphoblast, Left) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

    Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.

    Negative control: Daudi.

    Gated on viable cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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